当归MADS-box生物信息学及SOC1克隆与表达分析  

Analysis on MADS-box bioinformatics,SOC1 clone and expression pattern in Angelica sinensis

作　　者：崔秀文 刘迪 黄天苗 李美玲 栗孟飞[1] 魏建和[2] CUI Xiu-wen;LIU Di;HUANG Tian-miao;LI Mei-ling;LI Meng-fei;WEI Jian-he(State Key Laboratory of Aridland Crop Science,College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China)

机构地区：[1]甘肃农业大学生命科学技术学院,干旱生境作物学国家重点实验室,甘肃兰州730070 [2]中国医学科学院北京协和医学院,药用植物研究所,北京100193

出　　处：《中草药》2023年第5期1551-1560,共10页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(32160083);干旱生境作物学国家重点实验室(甘肃农业大学)基金项目(GSCS-2021-Z03);道地药材生态种植与质量保障项目(202103003);财政部和农业农村部:国家现代农业产业技术体系(CARS-21)。

摘　　要：目的对当归Angelica sinensis MADS-box基因家族进行筛选与生物信息学分析,并对SOC1-4基因进行克隆与表达验证。方法基于当归全长转录组筛选MADS-box基因家族,利用在线工具进行生物信息学分析,并利用qRT-PCR检测SOC1-4基因在不同材料中的表达水平。结果当归全长转录组中含有29个MADS-box基因家族成员,可分为10个亚家族,共含有6个保守基序,蛋白质序列长度为49~422 aa,相对分子质量为5697.56~49624.90,等电点为5.06~11.00,亚细胞结果显示当归MADS-box基因家族成员分别定位于叶绿体、细胞核、细胞质和线粒体,二级结构预测及3D建模显示同一亚家族结构相似;SOC1-4基因克隆片段与全长转录组测序结果一致;SOC1-4基因随种苗春化作用和植株发育时期延长呈现高表达,在抽薹植株、茎和叶中高表达,而在冷冻规避春化作用种苗中呈现低表达。结论当归含有29个MADS-box成员,各成员理化性质和结构存在一定的差异,SOC1-4基因表达水平与当归抽薹开花生理调控一致,为当归MADS-box基因家族调控抽薹开花相关研究提供借鉴。Objective To screen and analyze MADS-box gene family bioinformatics of Angelica sinensis,clone the SOC1-4 gene and perform bioinformatic analysis.Methods The MADS-box gene family was screened based on the full-length transcriptomes of A.sinensis,the bioinformatics was analyzed using the online tools,and the expression levels of SOC1-4 gene in different materials were validated by qRT-PCR.Results A total of 29 MADS-box gene family members were identified from A.sinensis,with 10 subfamily classified and six motifs included;the sequence length of MADS-box proteins ranged from 49 to 422 aa,the relative molecular mass ranges from 5697.56 to 49624.90,and the isoelectric point ranged from 5.06 to 11.00;the sub-cellular results showed that the members of the A.sinensis MADS-box gene family was located in the chloroplast,nucleus,cytoplasm and mitochondria;and secondary structure prediction and 3D modeling indicated that there was a similar structure for the same subfamily.The cloned fragment of the SOC1-4 gene was consistent with the full-length transcript.The SOC1-4 gene were over-expressed with the extension of seedlings vernalization and plants development,as well as in bolted plants,stems and leaves,respectively;while down-regulated in the seedlings with frozen-avoided vernalization.Conclusion There are 29 MADS-box gene family members in A.sinensis,and certain differences exist in physical and chemical properties and structure among the 29 members;the expression levels of SOC1-4 gene are in accordance with the physiological regulation of bolting and flowering of A.sinensis,The results will provide theoretical basis of MADS-box gene family for regulating bolting and flowering of A.sinensis.

关 键 词：当归 MADS-BOX 生物信息学 SOC1-4 基因克隆 抽薹开花 

分 类 号：R286.12[医药卫生—中药学]