AHP-CRITIC法结合正交设计优选丹参心脑通胶囊提取工艺  

Optimization of Extraction Process for Danshen Xinnaotong Capsules(丹参心脑通胶囊)Based on AHP-CRITIC Method Combined with Orthogonal Design

作　　者：郭丽 赵变[2] 吴作敏 王瑞 金少举[3] 于晓涛 GUO Li;ZHAO Bian;WU Zuomin;WANG Rui;JIN Shaoju;YU Xiaotao(The First People′s Hospital of Luohe/Henan Provincial Key Laboratory of Traditional Chinese Medicine Preparation and Processing,Luohe 462005,Henan,China;Department of Pharmacy,Luohe Medical College,Luohe 462000,Henan,China;Department of Pharmacology,Luohe Medical College,Luohe 462000,Henan,China)

机构地区：[1]漯河市第一人民医院药学部/河南省中药制剂与加工中医药重点实验室,河南漯河462005 [2]漯河医学高等专科学校药学系,河南漯河462000 [3]漯河医学高等专科学校药理学教研室,河南漯河462000

出　　处：《辽宁中医杂志》2023年第1期130-134,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：漯河市2022年度重大科技创新专项(揭榜挂帅)项目(漯科[2022]45号);漯河医学高等专科学校创新创业发展能力提升工程科研项目(2021LYZKJXM037)。

摘　　要：目的通过正交试验设计优选丹参心脑通胶囊的提取工艺,为其后续制备工艺的研究提供参考。方法采用HPLC-UV法测定丹酚酸B、葛根素的含量,采用反相高效液相色谱-蒸发光散射检测器(RP-HPLC-ELSD)法测定黄芪甲苷的含量,通过AHP-CRITIC法赋予指标性成分含量和干膏得率的权重系数,以综合评分为最终评价指标,优选提取工艺并进行验证试验。结果AHP-CRITIC法确定的权重系数科学、有效,优选的提取工艺条件为处方加8倍量水提取2次,每次1.5h。验证试验结果显示,综合评分均值为98.26(RSD=0.67%,n=3)。结论优选的提取工艺稳定性和重复性强,可以为丹参心脑通胶囊制备工艺的研究提供实验数据。Objective To optimize the extraction process of Danshen Xinnaotong Capsules(丹参心脑通胶囊)by orthogonal design to provide reference for its subsequent preparation process research.Methods The contents of salvianolic acid B and puera⁃rin were determined by HPLC-UV method,and the content of astragaloside IV was determined by RP-HPLC-ELSD method.The weight coefficients of the contents of salvianolic acid B,puerarin,astragaloside IV and dry paste yield were given by AHP-CRITIC method.The comprehensive score was taken as the final evaluation index,and the optimal extraction conditions were screened and verified.Results The weight coefficients determined by AHP-CRITIC method were scientific and effective.The op⁃timal extraction conditions were as follows:the prescription was extracted twice with 8 times of water,1.5 h for each time.Valida⁃tion test results showed that the mean comprehensive score was 98.26(RSD=0.67%,n=3).Conclusion The optimized extrac⁃tion process has strong stability and repeatability,which could provide theoretical basis for the subsequent preparation of Danshen Xinnaotong Capsules.

关 键 词：丹参心脑通胶囊 AHP-CRITIC法 正交设计 丹酚酸B 葛根素 黄芪甲苷 

分 类 号：R284.2[医药卫生—中药学]