牡丹促成栽培中糖信号调控成花的机理研究  

The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

作　　者：薛玉前 柳志勇 孙凯荣 张秀新[2] 吕英民[1] 薛璟祺[2] XUE Yuqian;IU Zhiyong;SUN Kairong;ZHANG Xiuxin;LÜYingmin;XUE Jingqi(College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,Institute of Vegetables and Flower,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]北京林业大学园林学院,北京100083 [2]中国农业科学院蔬菜花卉研究所,农业农村部园艺作物生物学与种质创制重点实验室,北京100081

出　　处：《园艺学报》2023年第3期596-606,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31972440,31972455);国家重点研发计划项目(2021YFE0110700);国家现代农业产业技术体系建设专项资金项目(CARS-21);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)

摘　　要：以牡丹栽培品种‘洛阳红’为试材,在促成栽培条件下对花芽进行剥叶和涂抹赤霉素处理,探究其对花芽发育、叶片气孔特征、葡萄糖、果糖、蔗糖和海藻糖-6-磷酸(T6P)水平以及糖信号相关基因表达模式等的影响。结果表明,剥叶和赤霉素处理均有效促进牡丹花蕾发育且能够显著促进叶片气孔的发育,提高气孔的开度;剥叶和赤霉素处理能够促进蔗糖和葡萄糖的代谢,并通过促进T6P的积累触发糖信号,最终通过上调PsTPS1及抑制PsSnRK1和PsHXK1在叶片中的表达,诱导花蕾发育。In this study,tree peony cultivar‘Luoyang Hong’was used as the material.Under the conditions of forcing culture,the flower buds were defoliated and treated with gibberellin to explore the effects on the development of flower buds,leaf stomatal characteristics,and glucose,fructose,sucrose,trehalose-6-phosphate(T6P)levels and the expression patterns of genes related to sugar signaling pathway.The results showed that both defoliation and gibberellin treatments can effectively promote the development of flower buds and can significantly promote the development of stomata and increase the opening of the stomata;at the same time,defoliation and gibberellin treatments could promote the metabolic consumption of energy substances,such as sucrose and glucose.The sugar signaling pathway was triggered by sensing the accumulation of sugar signaling molecule T6P content,and finally it promoted the development of flower bud by up-regulating the expression of PsTPS1 in leaves and inhibiting the expression of PsSnRK1 and PsHXK1.

关 键 词：牡丹 促成栽培 糖信号 海藻糖-6-磷酸 成花调控 

分 类 号：S685.11[农业科学—观赏园艺]