Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence  

作　　者：Leanne Maurice Alberto Bilenca 

机构地区：[1]Biomedical Engineering Department,Ben-Gurion University of the Negev,1 Ben Gurion Blvd,Be’er-Sheva,84105,Israel [2]Ilse Katz Institute for Nanoscale Science and Technology,Ben-Gurion University of the Negev,1 Ben Gurion Blvd,Be’er-Sheva,84105,Israel

出　　处：《Light(Science & Applications)》2023年第3期415-424,共10页光（科学与应用)（英文版）

基　　金：The authors thank Prof.Angel Porgador and Dr.Orly Gershoni-Yahalom of Ben-Gurion University of the Negev for the valuable assistance in the preparation of the biological samples.A.B.acknowledges the support of the Israel Science Foundation(grant no.1599/12).

摘　　要：Single particle tracking in three dimensions is an indispensable tool for studying dynamic processes in various disciplines,including material sciences,physics,and biology,but often shows anisotropic three-dimensional spatial localization precision,which restricts the tracking precision,and/or a limited number of particles that can be tracked simultaneously over extended volumes.Here we developed an interferometric,three-dimensional fluorescence single particle tracking method based on conventional widefield excitation and temporal phase-shift interference of the emitted,high-aperture-angle,fluorescence wavefronts in a greatly simplified,free-running,triangle interferometer that enables tracking of multiple particles at the same time with<10-nm spatial localization precision in all three dimensions over extended volumes(~35×35×2μm^(3))at video rate(25 Hz).We applied our method to characterize the microenvironment of living cells and up to~40μm deep in soft materials.

关 键 词：materials PARTICLE DIMENSIONS 

分 类 号：O657.3[理学—分析化学]