三疣梭子蟹卵巢发育过程中主要类胡萝卜素组成变化及其与抗氧化性能的关系  

CHANGES IN CAROTENOID COMPOSITION AND ANTIOXIDANT CAPACITY OF PORTUNUS TRITUBERCULATUS DURING OVARIAN DEVELOPMENT

作　　者：孙秋凤 柳梅梅 何杰[3] 张敏[1] 陶贤继[1,4,5] 吴旭干[1,4,5] SUN Qiu-Feng;LIU Mei-Mei;HE Jie;ZHANG Min;TAO Xian-Ji;WU Xu-Gan(Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affair,Shanghai Ocean University,Shanghai 201306,China;School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Marine and Fisheries Research Institute of Zhejiang Province,Zhoushan 316021,China;Shanghai Engineering Research Centre of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Centre for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学农业农村部鱼类营养和环境生态研究中心,上海201306 [2]江苏海洋大学海洋与水产学院,连云港222005 [3]浙江省海洋水产研究所,舟山316021 [4]上海海洋大学上海水产养殖工程技术研究中心,上海201306 [5]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《水生生物学报》2023年第5期713-722,共10页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2018YFD0900103);江苏省科技厅苏北科技专项(SZ-LYG202029);江苏省农业重大技术协同推广计划试点项目(2019-SJ-009-5)资助。

摘　　要：实验研究了三疣梭子蟹(Portunus trituberculatus)卵巢发育过程中(Ⅰ—V期)组织色泽、类胡萝卜素组成与含量、抗氧化和非特异性免疫指标的变化规律,进一步探讨了肝胰腺中类胡萝卜素含量变化与抗氧化指标的关系。结果表明:(1)卵巢发育期间,卵巢指数(GSI)显著增加(P<0.05),肝胰腺指数(HSI)整体呈先升后降的趋势;卵巢的红度(a^(*))值和黄度(b^(*))值、肝胰腺的亮度(L^(*))值和b^(*)值均呈显著上升趋势,但卵巢中L^(*)值呈下降趋势。(2)在卵巢发育过程中,卵巢中的总类胡萝卜素、虾青素、叶黄素和β-胡萝卜素含量均为先升后降的趋势,虾青素含量在Ⅳ期最高;肝胰腺中的虾青素含量呈上升趋势,而β-胡萝卜素含量为显著下降趋势;内表皮中的总类胡萝卜素、虾青素、叶黄素、海胆烯酮和β-胡萝卜素含量均呈现下降趋势;就不同组织中类胡萝卜素含量而言,内表皮中虾青素含量最高。卵巢和肝胰腺的L^(*)值与总类胡萝卜素含量呈显著负相关,卵巢的a^(*)值和b^(*)值均与总类胡萝卜素、虾青素、叶黄素、海胆烯酮和β-胡萝卜素含量呈显著正相关。肝胰腺的a^(*)值与各类胡萝卜素含量无显著相关性,b^(*)值仅与虾青素含量呈显著正相关。(3)肝胰腺中Ⅰ—V期T-AOC呈显著下降趋势(P<0.05),而SOD、CAT和GPX均为“先上升后下降”趋势;血淋巴中T-AOC呈现“高-低-高-低”的变化趋势,而SOD和GPX为“先上升后下降”趋势,CAT和MDA为“低-高-低-高”的变化趋势;卵巢发育过程中肝胰腺中ACP、ALP和NO整体呈下降趋势,而血淋巴中ACP、ALP和Hc在Ⅰ—Ⅳ期为上升趋势,V期略有下降。(4)相关性分析表明,肝胰腺中虾青素含量与T-AOC呈显著负相关,叶黄素含量与GPX呈显著正相关(P<0.01),其余类胡萝卜素含量与抗氧化指标均无显著相关性。综上,在三疣梭子蟹卵巢发育过程中,卵巢中总类胡萝卜素、虾青素和β-胡萝卜Female Portunus trituberculatus at different stages of ovarian development(stageⅠ—Ⅴ)were investigated for their color,carotenoids contents,antioxidant and non-specific immune parameters.The results indicated that:(1)the gonadosomatic index(GSI)increased significantly(P<0.05),while the hepatosomatic index(HSI)rose slightly,then declined gradually.The redness(a^(*))and yellowness(b^(*))values in ovaries showed an increasing trend,as did lightness(L^(*))and b^(*)values in hepatopancreas,but L^(*)values in ovaries showed a decreasing trend.(2)Total carotenoids,astaxanthin,zeaxanthin andβ-carotene content in ovaries showed an increasing trend followed by a decreasing trend,with the highest astaxanthin content in stage IV.Astaxanthin content in hepatopancreas was on the rise,whileβ-carotene was on a significant decline.Regarding the content of carotenoids in different tissues,the epithelium contained the highest amount of astaxanthin.L^(*)values in the ovary and hepatopancreas exhibited a significant negative correlation with total carotenoids,and a^(*)and b^(*)values in the ovary both showed a significant positive correlation with total carotenoids,astaxanthin,luteolin,echinenone andβ-carotene.There was no significant correlation between a^(*)values and various carotenoids in hepatopancreas,and b^(*)values only showed a significant positive correlation with astaxanthin.(3)For antioxidant indexes,T-AOC in hepatopancreas showed a significant decrease in stageⅠ—Ⅴ(P<0.05),while SOD,CAT and GPX showed an“increasing and then decreasing”trend.The T-AOC in hemolymph showed a trend of“high-low-high-low”.SOD and GPX showed an increasing trend and then decreasing trend,while CAT and MDA showed a“low-high-low-high”trend.In terms of immune indexes,ACP,ALP and NO in the hepatopancreas showed an overall decreasing trend during ovarian development,while ACP,ALP and Hc in the hemolymph showed an increasing trend in stageⅠ—Ⅳand a slight decrease in stageⅤ.(4)Correlation analysis showed that astaxanthi

关 键 词：性腺发育 类胡萝卜素 抗氧化性能 免疫性能 三疣梭子蟹 

分 类 号：Q175[生物学—水生生物学]