HDV RNA检测的研究现状  

Current research status of hepatitis D virus RNA detection

作　　者：高耀 任锋[1,2] 段钟平 GAO Yao;REN Feng;DUAN Zhongping(Beijing Municipal Key Laboratory of Liver Failure and Artificial Liver Treatment Research,Beijing YouAn Hospital,Capital Medical University,Beijing 100069,China;Beijing Institute of Hepatology,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京佑安医院,北京市肝衰竭与人工肝重点治疗研究重点实验室,北京100069 [2]北京肝病研究所,北京100069

出　　处：《临床肝胆病杂志》2023年第4期758-765,共8页Journal of Clinical Hepatology

基　　金：国家自然科学基金项目(81770611,82002243);北京自然科学基金和北京市教委联合资助重点项目(KZ202010025035);首都卫生发展科研专项重点攻关项目(首发2020-1-1151);北京市属医学科研院所公益发展改革试点项目(京医研2021-10);北京市卫健委高层次公共卫生技术人才建设项目“学科带头人”专项资助(SL-02-13);北京市医院管理中心“登峰人才”专项资助(DFL20221503);首都医科大学科研培育基金资助项目(PYZ22130)。

摘　　要：HDV感染导致的丁型肝炎是最严重的肝炎类型。由于缺乏精准HDV RNA检测方法,HDV的流行率被严重低估。HDV RNA是诊断HDV感染的金标准,在HDV的诊断、筛查和指导治疗方面具有重要意义。但因HDV基因型多、二级结构牢固,导致HDV RNA检测困难。本文就HDV RNA诊断方法的进展进行综述,阐述了HDV RNA检测方法从定性到定量检测的进展,为了解HDV RNA检测重要意义以及促进HDV RNA检测新方法的研发提供新思路。Hepatitis D caused by hepatitis D virus(HDV)infection is the most serious type of viral hepatitis.The prevalence rate of HDV has been seriously underestimated due to the lack of accurate HDV RNA detection methods.HDV RNA is the gold standard for the diagnosis of HDV infection and is of great significance in the diagnosis,screening,and treatment guidance of HDV.However,the multiple genotypes and strong secondary structure of HDV have led to great difficulties in HDV RNA detection.This article reviews the advances in HDV RNA detection methods and elaborates on the development from qualitative to quantitative detection methods,in order to provide new ideas for understanding the significance of HDV RNA detection and promoting the research and development of new HDV RNA detection methods.

关 键 词：δ肝炎病毒 RNA 诊断 

分 类 号：R440[医药卫生—诊断学] R373[医药卫生—临床医学]