复方灵丹胶囊对肝纤维化小鼠模型肝功能及纤维化程度的影响  

Effect of Compound Lingdan Capsule on liver function and fibrosis degree in mice with liver fibrosis

作　　者：涂芷馨 王妍[1] 黄剑洁 金清龙[1] TU Zhixin;WANG Yan;HUANG Jianjie;JIN Qinglong(Department of Hepatology,The First Hospital of Jilin University,Changchun 130000,China)

机构地区：[1]吉林大学第一医院肝胆胰内科,长春130000

出　　处：《临床肝胆病杂志》2023年第4期804-809,共6页Journal of Clinical Hepatology

基　　金：国家自然科学基金(81270484)。

摘　　要：目的研究复方灵丹胶囊对肝纤维化小鼠模型血清生化指标及肝脏纤维化程度的影响。方法125只SPF级雄性C57BL/6小鼠随机分为正常对照组(n=5);CCl 4模型组(n=15),CCl 4造模低、中、高剂量给药组(0.8、1.6、3.2 mg·g^(-1)·d^(-1))(每组各15只);DDC模型组(n=15),DDC造模低、中、高剂量给药组(0.8、1.6、3.2 mg·g^(-1)·d^(-1))(每组各15只)。造模成功后,给药组予以相应剂量复方灵丹胶囊混悬液灌胃,正常对照组及模型组以等量生理盐水灌胃,连续给药4周,眼球取血,留取血清,检测AST、ALT、白蛋白、胆红素,留取肝右叶同一部位组织进行病理观察及天狼星红、α1-Ⅰ型胶原蛋白抗体及α-平滑肌肌动蛋白抗体染色。计量资料多组间比较采用单因素方差分析,进一步两两比较采用Bonferroni法。结果与模型组对比,复方灵丹胶囊给药后,各剂量组小鼠血清ALT明显下降、白蛋白升高(P值均<0.05),中、高剂量给药组血清AST、胆红素水平均降低(P值均<0.05),且各指标高剂量组较低剂量组差异更显著(P值均<0.05)。各剂量组小鼠肝组织病理变化均得到不同程度的改善,天狼星红染色阳性细胞显著减少,α-平滑肌肌动蛋白、α1-Ⅰ型胶原蛋白表达出现不同程度的下降。结论复方灵丹胶囊能够改善肝纤维化小鼠肝功能、降低其肝纤维化程度。Objective To investigate the effect of Compound Lingdan Capsule on serum biochemical parameters and liver fibrosis degree in a mouse model of liver fibrosis.Methods A total of 125 specific pathogen-free male C57BL/6 mice were randomly divided into normal control group with 5 mice,CCl 4 model group with 15 mice,low-,middle-,and high-dose CCl 4 groups(0.8,1.6,and 3.2 mg·g^(-1)·d^(-1))with 15 mice in each group,DDC model group with 15 mice,and low-,middle-,and high-dose DDC groups(0.8,1.6,and 3.2 mg·g^(-1)·d^(-1))with 15 mice in each group.After successful modeling,the mice in the administration groups were given Compound Lingdan Capsule suspension at the respective doses by gavage,and those in the normal control group and the model group were given an equal volume of normal saline by gavage,for 4 consecutive weeks.Blood samples were collected from the eyeballs,and serum was used to measure aspartate aminotransferase(AST),alanine aminotransferase(ALT),albumin,and bilirubin.Liver tissue samples were collected at the same site of the right lobe of the liver for pathological observation,Sirius Red staining,α-SMA antibody staining,and COL1A1 antibody staining.A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the Bonferroni method was used for further comparison between two groups.Results Compared with the model group,each dose group had significant reductions in the serum level of ALT and a significant increase in the serum level of albumin after the administration of Compound Lingdan Capsule(all P<0.05),the levels of AST and bilirubin in the middle and high dose groups were lower(all P<0.05),and the difference of each index in the high dose group was more significant than that in the low dose group(all P<0.05).Each dose group had varying degrees of improvement in the pathological changes of the liver and a significant reduction in the number of Sirius Red staining-positive cells,as well as varying degrees of reduction in the protein expression ofα-SMA and COL1A1

关 键 词：肝硬化 小鼠 近交C57BL 复方灵丹胶囊 

分 类 号：R285.5[医药卫生—中药学]