生物安全柜防护性能评价用标准物质研制  

Development of Certified Reference Materials for Evaluation on Protection Performance of Biological Safety Cabinet

作　　者：王梓权 刘思渊 甄啸啸 黄文锋[1] 张玲[1] 徐倩[3,4] 隋志伟 WANG Zi-quan;LIU Si-yuan;ZHEN Xiao-xiao;HUNAG Wen-feng;ZHANG Ling;XU Qian;SUI Zhi-wei(Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029,China;Beijing Institute of Metrology,Beijing 100029,China;College of Life Sciences,Hunan Normal University,Changsha,Hunan 410081,China;Hunan Institute of Metrology and Test,Changsha,Hunan 410014,China)

机构地区：[1]中国计量科学研究院前沿科学计量中心,北京100029 [2]北京市计量检测科学研究院,北京100029 [3]湖南师范大学生命科学学院,湖南长沙410081 [4]湖南省计量检测研究院,湖南长沙410014

出　　处：《计量学报》2023年第3期334-340,共7页Acta Metrologica Sinica

基　　金：国家重点研发计划(2018YFC1200501);国家市场监督管理总局科技计划(2019MK114);中国计量科学研究院基本科研业务费(AKYYJ2009)。

摘　　要：生物安全柜的防护性能不仅影响实验结果的准确性和可靠性,而且也关系到实验人员的人身安全,因此其防护性能需要及时评价与校准。枯草芽孢杆菌作为国内外生物安全柜标准中规定的性能评价用指示菌,其芽孢标准物质的研制十分必要。通过将不同浓度的枯草芽孢杆菌芽孢添加到保护剂中,经冻干制备成干粉状标准物质,并研究标准物质的均匀性和稳定性。结果表明制备的枯草芽孢杆菌芽孢计数系列标准物质均匀性良好,在4℃下可以稳定保存24个月,3种不同浓度的标准物质标准值分别为(5.4±1.3)×10^(8)CFU/mL、(5.2±1.3)×10^(6)CFU/mL和(5.3±1.4)×10^(4)CFU/mL,可分别用于生物安全柜人员、产品和交叉污染保护性能评价。The protection performance of biosafety cabinet not only affects the accuracy and reliability of experimental results,but also relates to the personal safety of laboratory personnel,therefore its protection performance needs to be evaluated and calibrated in time.Bacillus subtilis(B.subtilis)is an indicator of performance evaluation specified in the domestic and international standards of biosafety cabinet,thus development of B.subtilis reference material is necessary.Three reference materials were prepared by freeze-drying B.subtilis spores in the presence of a composite cryoprotectant.The result indicated that the reference materials of B.subtilis spores count bearing excellent homogeneity and could be stored stably for twenty-four months at 4℃.The certified values of three different concentrations of certified reference materials were(5.4±1.3)×10^(8)CFU/mL,(5.2±1.3)×10^(6)CFU/mL,(5.3±1.4)×10^(4)CFU/mL,respectively.The reference materials of B.subtilis spores count could mainly be used to evaluate the staff,product,and cross-contamination protection of the biosafety cabinet.

关 键 词：计量学 生物安全柜 防护性能评价 枯草芽孢杆菌 芽孢 标准物质 

分 类 号：TB99[一般工业技术—计量学]