Nitroxoline Drug Combinations Are More Active Than Lyme Antibiotic Combination and Can Eradicate Stationary-Phase Borrelia burgdorferi  

作　　者：Hector S.Alvarez-Manzo Yumin Zhang Ying Zhang 

机构地区：[1]Department of Molecular Microbiology and Immunology,Bloomberg School of Public Health,Johns Hopkins University,Baltimore,Maryland,USA [2]State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang,China

出　　处：《Infectious Microbes & Diseases》2022年第3期124-130,共7页感染微生物与疾病（英文）

基　　金：supported by the Global Lyme Alliance,the Steven and Alexandra Cohen Foundation,the LivLyme Foundation,NatCapLyme,and the Einstein-Sim Family Charitable Fund.

摘　　要：Lyme disease(LD),caused by Borrelia burgdorferi,is themost common vector-borne disease in the United States and Europe.Despite the standard 2–4 weeks'antibiotic treatment,approximately 10%–20%of patients will develop posttreatment LD syndrome,a condition that is poorly understood.One of the probable causes is thought to be the presence of B.burgdorferi persister forms that are not effectively killed by the current LD antibiotics.In this study,we evaluated nitroxoline,an antibiotic used to treat urinary tract infections,for its activity against a stationary-phase culture enriched with persister forms of B.burgdorferi.Nitroxoline was found to be more active than doxycycline and equally active as cefuroxime(standard LD antibiotics)against B.burgdorferi.Importantly,the nitroxoline two-drug combinations nitroxoline+cefuroxime and nitroxoline+clarithromycin,as well as the nitroxoline three-drug combination nitroxoline+cefuroxime+clarithromycin,were as effective as the persister drug daptomycin-based positive control three-drug combination cefuroxime+doxycycline+daptomycin,completely eradicating stationary-phase B.burgdorferi in the drug-exposure experiments and preventing regrowth in the subculture study.Future studies should evaluate these promising drug combinations in a persistent LD mouse model.

关 键 词：Lyme disease Borrelia burgdorferi treatment nitroxoline drug repurposing 

分 类 号：R51[医药卫生—内科学]