The Utility of Voided Urine Samples as a Proxy for the Vaginal Microbiome and for the Prediction of Bacterial Vaginosis  

作　　者：Bin Zhu Christopher Diachok Laahirie Edupuganti David J.Edwards Jeffrey R.Donowitz Katherine Tossas Andrey Matveyev Katherine M.Spaine Vladimir Lee Myrna G.Serrano Gregory A.Buck 

机构地区：[1]Department of Microbiology and Immunology,School of Medicine,Virginia Commonwealth University,Richmond,Virginia,USA [2]Center for Microbiome Engineering and Data Analysis,Virginia Commonwealth University,Richmond,Virginia,USA [3]Department of Statistical Sciences and Operations Research,College of Humanities and Sciences,Virginia Commonwealth University,Richmond,Virginia,USA [4]Department of Pediatric Infectious Diseases,Children's Hospital of Richmond at Virginia Commonwealth University,Richmond,Virginia,USA [5]Department of Infectious Diseases and International Health,University of Virginia,Charlottesville,Virginia,USA [6]Department of Health Behavior and Policy,School of Medicine,Virginia Commonwealth University,Richmond,Virginia,USA [7]Department of Family Medicine and Population Health,School of Medicine,Virginia Commonwealth University,Richmond,Virginia,USA [8]Computer Science Department,College of Engineering,Virginia Commonwealth University,Richmond,Virginia,USA [9]Genomics Core,Virginia Commonwealth University,Richmond,Virginia,USA

出　　处：《Infectious Microbes & Diseases》2022年第4期149-156,共8页感染微生物与疾病（英文）

基　　金：supported by grants UH3AI083263,U54HD080784 and R01HD092415 fromthe National Institutes of Health and the GAPPS BMGF PPB grant from the Global Alliance to Prevent Prematurity and Stillbirth。

摘　　要：Recent work has shown that the vaginal microbiome exerts a strong impact on women's gynecological health.However,collection of vaginal specimens is invasive and requires previous clinical training or the involvement of a trained clinician.In contrast,urine sample collection is routine and noninvasive and does not require involvement of a clinician.We sought to compare the vaginal and urogenital microbiomes to assess the utility of voided urine samples as a proxy for the vaginal microbiome.Paired urogenital and vaginal samples were collected from pregnant women and characterized by 16S rRNA taxonomic profiling.We examined diversities and compositions of paired urogenital and vaginal microbiomes using five discrete strategies to explore the similarity between the vaginal and urogenital microbiomes.A strategy comparing the paired urogenital and vaginal microbiomes in which taxa were assigned using the STIRRUPS database and urine-specific taxa were removed showed no significant difference in diversity and composition between the paired urogenital and vaginal microbiomes.Moreover,the relative abundances of common vaginal taxa were linearly correlated with those in the paired urogenitalmicrobiomes.These similarities suggest that voided urine samples could represent a noninvasive protocol for accurate profiling of the vaginalmicrobiome with likely clinical applications.Finally,a machine learning model was established in which the voided urine microbiome was compared favorably to the vaginal microbiome in predicting bacterial vaginosis.

关 键 词：16S rRNA vaginal microbiome urine microbiome bacterial vaginosis reproductive and gynecologic health 

分 类 号：R711[医药卫生—妇产科学]