2株海鸭源H3亚型禽流感病毒全基因组分子特征分析  

Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province

作　　者：谢守玉 王睿敏 崔鹏飞[3] 施开创[1] 韦显凯[1] 冯淑萍[1] 龙凤 屈素洁[1] 陆文俊[1] 黄金山 黄凤梅 温新瑞 尹彦文[1] 邓国华[3] XIE Shouyu;WANG Ruimin;CUI Pengfei;SHI Kaichuang;WEI Xiankai;FENG Shuping;LONG Feng;QU Sujie;LU Wenjun;HUANG Jinshan;HUANG Fengmei;WEN Xinrui;YIN Yanwen;DENG Guohua(Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China;Guigang Center for Animal Disease Control and Prevention,Guigang 537100,China;Animal Influenza Laboratory of the Ministry of Agricultural and Rural Affairs,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China;Youjiang Town of Baise Center for Animal Disease Control and Prevention,Baise 533000,China;Qinnan Town of Qinzhou Center for Animal Disease Control and Prevention,Qinzhou 535000,China;Beihai Center for Animal Disease Control and Prevention,Beihai 536000,China)

机构地区：[1]广西动物疫病预防控制中心,南宁530001 [2]贵港市动物疫病预防控制中心,贵港537100 [3]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,农业农村部动物流感重点实验室,哈尔滨150069 [4]百色市右江区动物疫病预防控制中心,百色533000 [5]钦南区动物疫病预防控制中心,钦州535000 [6]北海市动物疫病预防控制中心,北海536000

出　　处：《中国畜牧兽医》2023年第4期1319-1328,共10页China Animal Husbandry & Veterinary Medicine

基　　金：广西自然科学基金(2020GXNSFBA297066);广西农业科技资助项目(Z201984、Z202032)。

摘　　要：【目的】了解广西沿海地区水禽H3亚型禽流感病毒的基因组分子特征。【方法】本研究通过RT-PCR一步法分段扩增法,对2020年从广西沿海地区分离到的2株海鸭源H3亚型禽流感病毒A/duck/Guangxi/S11359/2020(H3N1)与A/duck/Guangxi/S11374/2020(H3N8)进行全基因组测序与进化分析。【结果】2株H3亚型禽流感病毒的HA蛋白裂解位点序列均为340 PEKQTR↓GLFG 349,为低致病性禽流感病毒的分子特征,与受体嗜性关联的第226、228位氨基酸均为Q和T,可能仍主要结合禽类受体;BLAST分析结果显示,本研究H3N1亚型禽流感病毒的M、NP基因及H3N8亚型禽流感病毒的PA基因,与H7亚型禽流感病毒相似性最高,分别为99.5%、98.7%和98.9%,表明H3可能与H7亚型禽流感病毒发生了基因交换;2株H3亚型禽流感病毒与越南、孟加拉国、韩国等周边国家水禽源的H3、H4、H6、H7、H9等亚型相似性较高,可能拥有相同的进化来源;遗传进化分析表明,H3亚型禽流感病毒各基因进化分支均属于欧亚谱系,与水禽源毒株遗传距离较近,犬源、猫源的毒株次之,人源、猪源的毒株较远,但H3N1亚型禽流感病毒的NS基因与日本早期人源流感毒株A/Aichi/2/1968遗传关系最近,可能发生了基因置换。H3N8亚型禽流感病毒的HA基因检测有重组信号,重组的亲本毒株分别为A/duck/Beijing/40/04(相似性为93.1%)和A/canine/Beijing/20121215-34/2012(相似性为97.1%)。【结论】广西沿海地区的2株H3亚型禽流感病毒基因来源复杂,表现出遗传多样性特征。【Objective】This study was aimed to better understand the molecular characteristics of complete genomes of waterfowl infected with H3 subtype Avian influenza virus(AIV)in the coast area of Guangxi.【Method】Two strains of H3 subtype AIV named A/duck/Guangxi/S11359/2020(H3N1)and A/duck/Guangxi/S11374/2020(H3N8)were acquired by one-step RT-PCR amplification of genome segment,then conducted sequencing and evolutionary analysis further in this study.【Result】The amino acid cleavage sites of HA protein of both H3 subtypes AIV were 340 PEKQTR↓GLFG 349 each,which was in accordance with the molecular characteristics of low pathogenic AIV,and the 226th and 228th amino acid sites correlated to receptor tropism were Q and T,indicating that were poultry receptor as possible.The BLAST analysis result showed that M and NP genes of H3N1 and PA gene of H3N8 with the highest similarity to H7 subtype AIV was 99.5%,98.7%and 98.9%,respectively,which suggested that genes recombination was occurred between H3 and H7 subtypes AIV.The genes of both H3 subtype AIV shared the highest similarity with that of waterfowl-origin H3,H4,H6,H7 and H9 subtypes AIV from Vietnam,Bangladesh and South Korea countries around China,which indicated that the isolates possessed the same evolutionary source.Genetic analysis indicated that all genes of both H3 subtype AIV isolates belonged to the Eurasian branch,the genetic distance of those was the closest to waterfowl-origin influenza virus strains,closer to canine and feline-origin strains,close to human and porcine-origin strains,but in addition to NS gene of H3N1 isolate which got the closest genetic distance to A/Aichi/2/1968 derived from Japanese earlier,suggesting that genes exchanged could occurred between them.Recombination sign of HA gene of H3N8 isolate was detected,the recombinant parent strains of that were A/duck/Beijing/40/04 and A/canine/Beijing/20121215-34/2012 strains,with the similarity was 93.1%and 97.1%,respectively.【Conclusion】The source and genetic characteristics of gen

关 键 词：海鸭 H3亚型禽流感病毒 遗传进化 

分 类 号：S852.65[农业科学—基础兽医学]