松辽黑猪FOXA 2基因第3外显子多态性及其与繁殖性状的关联分析  

Polymorphism of FOXA 2 Gene Exon 3 and Its Association with Reproductive Traits in Songliao Black Pigs

作　　者：张方玮 张琪[1] 张云鹏 李欣 张净搏 张树敏 于永生[1] ZHANG Fangwei;ZHANG Qi;ZHANG Yunpeng;LI Xin;ZHANG Jingbo;ZHANG Shumin;YU Yongsheng(Institute of Animal Biotechnology,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Gongzhuling National Agricultural Science and Technology Park Feimas Animal Husbandry Co.,Ltd.,Gongzhuling 136100,China)

机构地区：[1]吉林省农业科学院动物生物技术研究所,公主岭136100 [2]吉林农业大学动物科学技术学院,长春130118 [3]公主岭国家农业科技园区飞马斯牧业有限公司,公主岭136100

出　　处：《中国畜牧兽医》2023年第4期1444-1451,共8页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省科技厅重点研发项目(20220202039NC)。

摘　　要：【目的】分析叉头框家族转录因子A2(forkhead box A2,FOXA2)基因第3外显子多态位点在地方猪种中的遗传多样性及其与松辽黑猪繁殖性状的相关性。【方法】采集90头健康经产松辽黑猪母猪耳组织,采用直接测序法检测FOXA 2基因第3外显子SNP位点,利用SPSS 26.0软件分析FOXA 2基因第3外显子SNP位点多态性(基因型频率、基因频率、遗传纯合度、遗传杂合度、有效等位基因数、多态信息含量)及其与松辽黑猪母猪繁殖性状(总产仔数、产活仔数、乳头数以及仔猪初生重、3周龄重、断奶重、断奶仔猪数)的相关性。【结果】松辽黑猪FOXA 2基因第3外显子共检测出3个SNPs位点,1337 bp处SNP位点(C1337A)上发现CC、CA和AA 3种基因型;1343 bp处SNP位点(C1343G)上发现CC、CG和GG 3种基因型;1626 bp处SNP位点(C1626T)上发现CC、CT和TT 3种基因型。卡方适合性检验表明,松辽黑猪FOXA 2基因第3外显子的C1337A、C1626T位点符合哈迪-温伯格平衡状态(P>0.05),C1343G位点偏离哈迪-温伯格平衡状态(P<0.05)。多态信息含量(PIC)计算显示,C1337A、C1626T位点为中度多态(0.25<PIC<0.50),C1343G位点为高度多态(PIC>0.50)。关联分析结果表明,C1337A位点的CC基因型个体总产仔数显著高于CA基因型(P<0.05),AA基因型个体所产仔猪3周龄重、断奶重极显著高于CC、CA基因型(P<0.01);C1626T位点的CC基因型个体所产仔猪3周龄重、断奶重极显著或显著高于CT、TT基因型(P<0.01;P<0.05),CC基因型个体乳头数显著高于CT基因型个体(P<0.05)。【结论】FOXA 2基因第3外显子存在3个多态性位点,与松辽黑猪总产仔数、3周龄重、断奶重和乳头数均有显著关联性。【Objective】The aim of this study was to analyze the genetic diversity of forkhead box A2(FOXA2)gene exon 3 polymorphism in local pig breeds and its correlation with reproductive traits in Songliao Black pigs.【Method】The single nucleotide polymorphisms(SNPs)(genotype frequency,gene frequency,genetic homozygosity,genetic heterozygosity,number of effective alleles,polymorphic information content)of FOXA 2 gene exon 3 were screened by direct sequencing in ear tissues of 90 healthy Songliao Black pigs,and the correlation between SNP of FOXA 2 gene eoxn 3 and reproductive performances(total number of litter,number born alive,number of nipples,birth weight,3-week weight,weaning weight and weaned piglets)were analyzed using SPSS 26.0 software.【Result】The results obtained that SNPs sites(C1337A,C1343G,C1626T)were found of FOXA 2 gene exon 3 in Songliao Black pig.Genotypes of CC,CA and AA were contained in C1337A,genotypes of CC,CG and GG were contained in C1343G,and genotypes of CC,CT and TT were contained in C1626T.The chi-square fitness test showed that SNPs(C1337A,C1626T)were in Hardy-Weinberg equilibrium(P>0.05),C1343G was not in Hardy-Weinberg equilibrium(P<0.05).The calculation of polymorphism information content(PIC)showed that the genetic heterozygosities of C1337A and C1626T were in moderate polymorphism(0.25<PIC<0.50),the genetic heterozygosity of C1343G was in the highly polymorphism(PIC>0.50).The results of association analysis showed that the total number born of CC genotype with C1337A locus was significantly higher than that of CA genotype(P<0.05),the 3-week weight and weaning weight of piglets born by AA genotype individuals were extremely significantly higher than that of CC and CA genotype(P<0.01);The 3-week weight and weaning weight of piglets born by CC genotype individuals with C1626T locus were significantly or extremely significantly higher than that of CT and TT genotype(P<0.05 or P<0.01),the number of nipples of CC genotype was significantly higher than that of CT genotype(P<0.05).【C

关 键 词：松辽黑猪 FOXA 2基因 第3外显子 繁殖性状 关联分析 遗传标记 

分 类 号：S813.3[农业科学—畜牧学]