丁酸梭菌和丁酸钠对高脂饮食诱导雄性小鼠生殖损伤的影响  

Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice

作　　者：杜朝晖 葛梓豪 游君怡 刘晓玮 梁国栋 孙世铎[1] 胡建宏[1] 李晓[1] DU Zhaohui;GE Zihao;YOU Junyi;LIU Xiaowei;LIANG Guodong;SUN Shiduo;HU Jianhong;LI Xiao(College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China;Dali Zhongkang Livestock Breeding Co.,Ltd.,Dali 715100,China)

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]大荔县众康家畜良种繁育有限公司,大荔715100

出　　处：《中国畜牧兽医》2023年第4期1603-1616,共14页China Animal Husbandry & Veterinary Medicine

基　　金：陕西省重点研发计划项目(2022ZDLNY01-05);陕西省生猪产业体系。

摘　　要：【目的】探讨丁酸梭菌和丁酸钠对高脂饮食刺激下小鼠精液品质和睾丸组织的影响,为治疗雄性生殖疾病提供试验基础和理论支持。【方法】试验1:将32只8周龄C57BL/6雄性小鼠随机均分为4组,对照组(NC)饲喂基础饲粮,高脂组(HFD)、丁酸梭菌处理组(HFD+C.butyricum)和丁酸钠处理组(HFD+Sodium butyrate)均饲喂含60%脂肪的高脂饲料,对照组和高脂组灌胃生理盐水,丁酸梭菌处理组灌胃1×108 CFU/kg丁酸梭菌,丁酸钠处理组灌胃300 mg/kg丁酸钠,每天1次。试验2:将16只8周龄C57BL/6雄性小鼠随机均分为2组,对照组正常饮食补充生理盐水,试验组小鼠通过灌胃补充1×108 CFU/kg丁酸梭菌溶液,每天1次,12周后处死采样。利用精液检测系统CASA检测精液品质,HE染色观察睾丸组织病理变化;利用试剂盒检测睾丸组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)、血清睾酮(T)含量,以及睾丸组织碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)及γ-谷氨酰基转移酶(γ-GT)活性。【结果】试验1中,与高脂组相比,丁酸梭菌处理组小鼠体重极显著降低(P<0.01),小鼠精子活力、A+B级精子数、精子直线运动能力、平均路径速度均显著提高(P<0.05),直线速度极显著提高(P<0.01),小鼠睾丸组织AKP活性显著提高(P<0.05);丁酸钠处理组小鼠体重极显著降低(P<0.01),睾丸指数、精子直线运动能力、精子向前运动力、直线速度和摆动性均显著提高(P<0.05),精子活力、A+B级精子数、平均路径速度及睾丸组织γ-GT活性均极显著提高(P<0.01),此外,丁酸钠还显著增加了高脂小鼠血清睾酮含量、ACP活性(P<0.05),显著降低了小鼠睾丸组织MDA含量(P<0.05)。睾丸组织病理切片显示,各组小鼠睾丸组织形态并无明显差异。试验2中,与对照组相比,试验组小鼠睾丸指数极显著升高(P<0.01),血清中睾酮水平显著升高(P<【Objective】This study was aimed to investigate the effects of Clostridium butyrate(C.butyricum)and sodium butyrate on semen quality and testicular tissue of mice stimulated by high fat diet,so as to provide experimental basis and theoretical support for the treatment of male reproductive diseases.【Method】In experiment 1,328-week-old C57BL/6 male mice were randomly divided into 4 groups.The mice in control group(NC)were fed a basal diet,in high fat diet group(HFD),C.butyricum treatment group(HFD+C.butyricum)and sodium butyrate treatment group(HFD+Sodium butyrate)were fed high fat diet containing 60%fat.HFD+C.butyricum group was gavaged with 1×108 CFU/kg C.butyricum,while HFD+Sodium butyrate group was gavaged with 300 mg/kg sodium butyrate,once a day.In experiment 2,168-week-old C57BL/6 male mice were randomly divided into two groups.The normal diet of control group was supplemented with normal saline,and the experiment group was supplemented with C.butyricum solution 1×108 CFU/kg by gavage,once a day.After 12 weeks,the animals were sacrificed for sampling.The semen quality was detected by CASA,and the pathological changes of testicular tissue were observed by HE staining.The total antioxidant capacity(T-AOC),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),the content of malondialdehyde(MDA)in testicular tissue and serum testosterone(T),and the activities of alkaline phosphatase(AKP),acid phosphatase(ACP),lactate dehydrogenase(LDH)and gamma glutamyltransferase(γ-GT)in testicular tissue were detected by corresponding kits.【Result】In experiment 1,compared with high-fat group,the body weight of mice in C.butyricum treatment group was extremely significantly decreased(P<0.01),the sperm motility,the number of A+B grade sperm,sperm linear motility and the velocity of average path of mice were significantly increased(P<0.05),and the straight-line velocity of mice was extremely significantly increased(P<0.01).The activity of AKP in testicular tissue of mice was significantly inc

关 键 词：丁酸梭菌 丁酸钠 高脂 睾丸 精液品质 

分 类 号：S816.73[农业科学—饲料科学]