铁蛋白结构和功能的生物信息学分析  

Bioinformatics analysis of the structure and function of ferritin

作　　者：窦承贤 方敏全 梁晋涛[1] 李桂银[1,2] DOU Cheng-xian;FANG Min-quan;LIANG Jin-tao;LI Cui-yin(School of Life and Environmental Sciences,Guilin University of Electronic Technology,Guilin 541004,China;College of Chemistry,Guangdong University of Petrochemical Technology,Maoming 525000,China)

机构地区：[1]桂林电子科技大学生命与环境科学学院,广西桂林541004 [2]广东石油化工学院化学学院,广东茂名525000

出　　处：《生物技术》2023年第1期33-37,67,共6页Biotechnology

基　　金：国家自然科学基金项目(62161009)。

摘　　要：[目的]通过生物信息学方法探究铁蛋白的结构和功能的相关信息,研究铁蛋白与肝癌的潜在关系。[方法]从NCBI获取铁蛋白序列,分别使用ProScale、TMHMM、NCBI-CDD和Prosite工具分析铁蛋白的亲疏水性、跨膜区、功能结构域和生物活性位点。使用SOPMA、PSIPRED和SWISS-MODEL平台预测了铁蛋白的二级结构和三级结构。进一步,基于STRING数据库研究了铁蛋白的互作蛋白和富集通路。[结果]铁蛋白是一种亲水性蛋白,无跨膜区,存在1个铁蛋白样二铁氧化的功能结构域和2个生物活性位点,具有较高的螺旋程度。铁蛋白与RPS17、TFRC、SCARA5、NCOA4和COX8A五种蛋白相互作用,这些蛋白显著富集在铁死亡与矿物吸收富集途径,与癌症的发生有关。[结论]对铁蛋白进行生物信息学分析,预测了铁蛋白重链和轻链的理化性质和空间结构,也从其功能上揭示了与肝癌的潜在关系,为深入研究提供了理论基础。[Objective]Through bioinformatics methods to explore the relevant information of the structure and function of ferritin, and studied the potential relationship between ferritin and liver cancer.[Method]The amino acid sequence of ferritin was obtained from NCBI.The hydrophilicity, transmembrane region, functional domain and bioactive site of ferritin were analyzed by ProScale, TMHMM,NCBI-CDD and Prosite tools respectively.SOPMA and SWISS-MODEL tools visualized the secondary and tertiary structures of ferritin.Further, the interaction proteins and enrichment pathways of ferritin were studied based on string database.[Result]Ferritin was a hydrophilic protein without transmembrane region.There was a function domain of ferritin-like diiron domin profile and two bioactive sites, which had a high degree of helicity.Ferritin interacted with RPS17,TFRC,SCARA5,NCOA4 and COX8A.These proteins were significantly enriched in the pathway of iron death and mineral absorption and enrichment, which were related to the occurrence of a variety of cancers.[Conclusion]The bioinformatics analysis of ferritin was carried out, the physicochemical properties and spatial structure of FTH1 and FTL were predicted, and the potential relationship with liver cancer was revealed from its function, which provided a theoretical basis for in-depth research.

关 键 词：铁蛋白 重链 轻链 肝癌 互作蛋白 铁死亡 

分 类 号：Q71[生物学—分子生物学]