三氯生对黄河鲤幼鱼性腺发育分化相关基因表达的影响  

Effects of Triclosan on Gene Expression Related to Gonadal Development and Differentiation in Juvenile Yellow River Carp(Cyprinus carpio)

作　　者：王凡[1] 刘雪琴[1] 刘飞[1] 冉娜娜 高晓杨 石亚男 朱娜娜 程惠敏 杨瑞 WANG Fan;LIU Xue-qin;LIU Fei;RAN Na-na;GAO Xiao-yang;SHI Ya-nan;ZHU Na-na;CHENG Hui-min;YANG Rui(Luoyang Normal University,Luoyang 471934,P.R.China)

机构地区：[1]洛阳师范学院,河南洛阳471934

出　　处：《水生态学杂志》2023年第2期114-119,共6页Journal of Hydroecology

基　　金：国家自然科学基金(31971524);NSFC-河南人才培养联合基金(U1504303);河南省高等学校青年骨干教师培养计划项目(2016-GGJS-119)。

摘　　要：为了探讨三氯生(TCS)对黄河鲤(Cyprinus carpio)幼鱼性腺分化的分子机制,采用半静态水体暴露法,将体长为7.8~9.9 cm的黄河鲤幼鱼暴露于0(对照)、0.04、0.08、0.16 mg/L的三氯生溶液中42 d,利用RT-qPCR技术检测黄河鲤性腺发育分化相关基因表达情况。结果显示,雄鱼精巢中,dax1和sox9a基因在0.04 mg/L和0.08 mg/L TCS处理组中均极显著上升(P<0.01),0.08 mg/L处理组中sox9b、dmrt1和foxl2基因显著或极显著上升(P<0.01,P<0.05,P<0.01),nobox基因在0.04 mg/L和0.16 mg/L TCS处理组显著和极显著下降(P<0.05,P<0.01),zp2基因在各TCS处理组显著或极显著上升(P<0.05,P<0.01),ar和amh基因在各TCS处理组显著和极显著下降(P<0.05,P<0.01);雌鱼卵巢中,sox9b基因在0.04 mg/L和0.08 mg/L TCS处理组均极显著上升(P<0.01),sox9a和dax1基因在0.04 mg/L TCS处理组显著和极显著上升(P<0.05,P<0.01),zp2基因在TCS处理组显著或极显著下降(P<0.05,P<0.01),foxl2基因在0.04 mg/L TCS处理组显著下降(P<0.05),nobox基因在0.16 mg/L TCS处理组显著下降(P<0.05)。研究表明,TCS影响了黄河鲤幼鱼性腺发育分化相关基因的表达,可能进一步影响其生殖腺的发育分化。In recent years,monitoring has shown varying levels of triclosan(TCS)pollution in the Yel⁃low River,high enough to threaten the aquatic ecosystem and human health along the Yellow River.Previous research has shown that TCS exposure affects the sexual differentiation of Yellow River carp(Cyprinus carpio).In this study,we investigated gene expression related to gonadal development and differentiation in C.carpio using a semistatic water contamination method and RT-PCR,and the effect of TCS on the molecular mechanism of gonadal differentiation of juvenile Yellow River carp was analyzed.The study aimed to provide a reference for determining environmental standards and evaluating ecological health.Healthy C.carpio of body length 7.8-9.9 cm were acclimated for 7 d in aquaria,and then exposed to four TCS concentrations(0,0.04,0.08,and 0.16 mg/L)for 42 days.Half of the solution was changed every 24 hours.Then the expression levels were determined for the genes amh,dax1,dmrt1,foxl2,nobox,sox9a,sox9b,ar,zp2 andβ-actin in C.carpio gonads by RT-PCR.Results were as follows:(1)In the sperm of male juveniles,expression of the genes dax1 and sox9a increased significantly in the 0.04 and 0.08 mg/L TCS treatments(P<0.01);the expression of the genes sox9b,dmrt1 and foxl2 increased significantly in the 0.08 mg/L TCS group(P<0.01,P<0.05,P<0.01);nobox gene expression significantly de⁃creased in the 0.04 and 0.16 mg/L TCS groups(P<0.05,P<0.01);zp2 gene expression significantly increased,and ar and amh gene expression significantly decreased in all TCS groups(P<0.05,P<0.01).(2)In the ovaries of female juveniles,sox9b gene expression significantly increased in the 0.04 and 0.08 mg/L TCS groups(P<0.01);sox9a and dax1 gene expression significantly increased(P<0.05,P<0.01)and foxl2 gene expression significantly decreased in the 0.04 mg/L TCS group(P<0.05);nobox gene expression decreased significantly in the 0.16 mg/L TCS group(P<0.05);and zp2 gene expression de⁃creased significantly in all the TCS groups(P<0.05,P<0.01).TCS has a sign

关 键 词：三氯生 黄河鲤 性腺发育分化 基因表达 

分 类 号：Q503[生物学—生物化学]