基于明胶及其衍生物/甲基丙烯酸羟乙酯/硫酸钠的形状记忆水凝胶  

Shape Memory Hydrogel Based on Gelatin and Its Derivatives/Hydroxyethyl Methacrylate/Sodium Sulfate

作　　者：何苗苗 陈凌东 张莉 甄辉 徐萍华 鲍娇慧 HE Miaomiao;CHEN Lingdong;ZHANG Li;ZHEN Hui;XU Pinghua;BAO Jiaohui(Zhejiang Institute of Medical Device Supervision and Testing,Hangzhou 310018,China)

机构地区：[1]浙江省医疗器械检验研究院,杭州310018

出　　处：《功能高分子学报》2023年第2期178-184,共7页Journal of Functional Polymers

基　　金：浙江省重点研发项目(2021C03035);浙江省属科研院所扶持经费项目(20220004)。

摘　　要：以明胶(Gel)、甲基丙烯酸羟乙酯(HEMA)为单体,以甲基丙烯酰化明胶(GelMA)为交联剂,通过光聚合法及Hofmeister效应制备出一种双网络(DN)水凝胶,光照结束后,将DN水凝胶浸泡到高浓度Na2SO4溶液中,得到DN/SO_(4)^(2-)水凝胶。采用万能材料试验机测试水凝胶的力学性能,采用MTT法对水凝胶的生物相容性进行表征。结果表明:当Gel质量分数为20%,HEMA质量分数为40%,GelMA质量分数为10%时,水凝胶的拉伸强度最高可达0.94 MPa,断裂伸长率为496%。水凝胶网络中的分子链在高浓度Na_(2)SO_(4)溶液中收缩,在低浓度Na_(2)SO_(4)溶液中舒展,赋予水凝胶离子刺激响应的形状记忆性。此外,该水凝胶还具备生物可降解性和良好的生物相容性。Double network(DN)hydrogel was prepared by photoinitiated polymerization with gelatin(Gel)and hydroxyethyl methacrylate(HEMA)as monomers,methacryloylated gelatin(GelMA)as a cross-linking agent,and 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylphenylacetone(I2959)as an initiator.Then the DN hydrogel was soaked into saturated Na2SO4 solution to prepare DN/SO_(4)^(2−)hydrogel.Tensile property was tested by universal material testing machine.Results showed that when the mass fraction of Gel,HEMA,GelMA was 20%,40%,and 10%,respectively,and the immersion time was 16 h,the tensile strength of the hydrogel could reach 0.94 MPa,and the elongation at break was 496%.Loading-unloading test showed that the hydrogel had good self-recovery performance.The hydrogel also had biodegradability because of the degradable cross-linking agent GelMA.It could be completely degraded after soaking in trypsin solution for 4 h.The molecular chains in the hydrogel network contracted in the high mass fraction salt solution and stretched in the low mass fraction salt solution,giving the shape memory of the hydrogel by ion stimulation response.The hydrogel with fixed shape(knotting,folding,twisting)could be recovered to its original shape within 5 min after soaking in low mass fraction Na_(2)SO_(4) solution(w=2%).In addition,nontoxic components endowed the hydrogel with excellent biocompatibility,which had been confirmed by MTT method.After co-culture with different mass concentrations of DN/SO_(4)^(2−)hydrogel extract solution,the viabilities of L929 cells were more than 80%.

关 键 词：双网络水凝胶 力学性能 形状记忆 生物可降解 生物相容性 

分 类 号：O63[理学—高分子化学]