2005-2020年转Bt基因棉花抗虫性变化及其与产量性状的相关性分析  

Changes of Insect Resistance and Its Correlation Analysis with Yield Traits in Transgenic Bt Cotton from 2005 to 2020

作　　者：唐中杰[1] 谢德意[1] 许守明 聂利红[1] 吕淑平[1] 王明坤[3] Tang Zhongjie;Xie Deyi;Xu Shouming;Nie Lihong;LüShuping;Wang Mingkun(Institute of Industrial Crops,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;State Key Laboratory of Cotton Biology,Henan University,Kaifeng 475004,Henan,China;Dengzhou Agricultural Technology Extension Center,Dengzhou 474150,Henan,China)

机构地区：[1]河南省农业科学院经济作物研究所,河南郑州450002 [2]河南大学棉花生物学国家重点实验室,河南开封475004 [3]邓州市农业技术推广中心,河南邓州474150

出　　处：《作物杂志》2023年第2期77-82,共6页Crops

基　　金：国家现代农业产业技术体系——棉花产业技术体系(CARS-18-04)。

摘　　要：为了给转Bt基因棉花在科研和生产上多世代可持续利用提供依据,以转Bt基因棉花品种银山8号为试验材料,从2005年开始,连续16年进行跟踪研究。结果表明,随种植世代的增加,生物测定银山8号第2~4代棉铃虫幼虫校正死亡率、铃期叶片和铃期小铃杀虫蛋白表达量均有不同程度的线性上升趋势,同时皮棉产量线性回归也呈增长趋势;苗期叶片、蕾期叶片和蕾期小蕾杀虫蛋白表达量呈线性下降趋势。相关性分析表明,抗虫棉的抗虫性与皮棉产量间均呈正相关,株铃数和衣分是构成皮棉产量的重要因子,与皮棉产量的相关性分别达到极显著和显著水平。外源Bt基因转入棉花后能稳定遗传给后代,通过卡那霉素鉴定和系统选择可以持续保持转Bt基因棉花多世代的抗虫性,甚至通过提纯复壮逐年优选,可实现转Bt基因抗虫棉特定生育时期或部分棉花器官抗虫性的提高。To provide references for the sustainable use of transgenicBt cotton in both scientific research and production,this study recorded Yinshan 8 of transgenic Bt cotton for 16 consecutive years since 2005.The results indicated that with the increase of planting-generations,the adjusted mortality of the second,third,and fourth generations of cotton bollworm larvae,and the expression of insecticidal protein of leaves and bolls at bolling stage of Yinshan 8 showed a linear increase in varying degrees,and an increasing trend was also observed for the linear regression of lint yield.The expression of insecticidal protein in leaves at the seedling stage,and in leaves and buds at the bud stage decreased linearly.Correlation analysis suggested that there was a positive correlation between the resistance of insect-resistant cotton and lint yield.The boll number and ginning outturn were important factors constituting the lint yield,which reached extremely significant and significant levels,respectively.The exogenous Bt gene could be stably passed to offspring after being transferred into cotton.Through kanamycin-based identification and systematic selection,the insect-resistance phenotype of transgenicBt cotton could be maintained for multiple generations.Through purification and rejuvenation,it was even possible to improve the insect resistance of the transgenicBt cotton in a specific growth period or in certain organs.

关 键 词：转BT基因棉花 抗虫性 产量性状 相关性分析 

分 类 号：S562[农业科学—作物学]