水稻潜根线虫生防菌筛选、鉴定及其杀线虫活性研究  

Screening and Identification of Biocontrol Fungi againstHirschmanniella sp. and Analysis of Nematocidal Activity

作　　者：贾路明 莫爱素 莫意雪 吴海燕[1] Jia Luming;Mo Aisu;Mo Yixue;Wu Haiyan(Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety/Agricultural College of Guangxi University,Nanning 530004,Guangxi,China)

机构地区：[1]广西农业环境与农产品安全重点实验室/广西大学农学院,广西南宁530004

出　　处：《作物杂志》2023年第2期238-244,共7页Crops

基　　金：国家自然科学基金(31460464)。

摘　　要：水稻潜根线虫在世界各稻区均普遍发生,给水稻生产造成一定的经济损失。从前期对根结线虫有较好毒杀活性的9个菌株中筛选出对水稻潜根线虫有很好毒杀效果的菌株。结果表明,2周发酵液2.5倍稀释液处理水稻潜根线虫,菌株ZW-1和XO-2对潜根线虫毒杀活性较强,72h的死亡率分别为93.7%和96.7%,LC_(50)值分别为90.4451和76.3875μL/mL。根据形态学特征和rDNA-ITS序列分析,鉴定菌株ZW-1和XO-2分别为日本曲霉(Aspergillus japonicus)和黑曲霉(A.niger)。这些生防真菌菌株的发掘,为植物线虫病害的生物防治提供了新的微生物资源。Hirschmanniella sp.is common in all rice regions of the world,which causes some economic losses of rice production.From the nine strains with good virulence against root-knot nematodes,we selected the strains with good virulence against of rice latent root nematodes.The results showed that ZW-1 and XO-2 had high nematocidal activities againstHirschmanniella sp.,and the mortality rate in 2.5×dilution for 72h were 93.7%and 96.7%,respectively,with LC_(50) values of 90.4451 and 76.3875μL/mL,respectively.Strains ZW-1 and XO-2 were identified asAspergillusjaponicus andA.niger,respectively,based on morphological and rDNA-ITS molecular analysis.The discovery of two biocontrol fungal strains provides new microbial resources for the biological control of plant nematode diseases.

关 键 词：水稻潜根线虫 生防菌 筛选 杀线虫活性 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]