苦荞醋饮的安全性初步研究  

Preliminary study on safety of tartary buckwheat vinegar drink

作　　者：张昕 王晨尧 司霞 朱瑞芳[1,2] 曹妍 冯耀清 韩世范 ZHANG Xin;WANG Chenyao;SI Xia;ZHU Ruifang;CAO Yan;FENG Yaoqing;HAN Shifan(Nursing College of Shanxi Medical University,Shanxi 030001 China)

机构地区：[1]山西医科大学护理学院,山西030001 [2]山西医科大学第一医院 [3]山西医学期刊社有限责任公司

出　　处：《护理研究》2023年第7期1232-1240,共9页Chinese Nursing Research

摘　　要：目的:研究药食两用物质苦荞发酵而成的苦荞醋饮有无急性经口毒性和亚急性经口毒性,为安全的食品提供动物实验依据。方法:在急性经口毒性试验中,剂量组灌胃人体推荐摄入量100倍的苦荞醋饮,对照组灌胃蒸馏水,灌胃体积为20 mL/kg,灌胃后连续观察14 d,记录小鼠的一般情况、体重变化、大体解剖检查结果及脏器系数等数据;在28 d经口毒性试验中,高、中、低剂量组分别灌胃人体推荐摄入量100倍、50倍、25倍的苦荞醋饮,对照组给予蒸馏水,连续灌胃28 d,灌胃体积为10 mL/kg,试验期间记录大鼠的一般情况、体重变化、摄食量和食物利用率等数据,试验结束时进行血液学检查、血生化检查、大体解剖检查及脏器系数的测定、组织病理学观察。结果:小鼠未出现中毒症状,也未出现死亡。与对照组相比,剂量组小鼠的体重、主要器官的脏器系数差异均无统计学意义(P>0.05);大鼠未出现中毒表现,也未出现死亡,低剂量组雌性大鼠的白细胞计数、中剂量组雄性大鼠的脾脏绝对重量与对照组比较差异有统计学意义(P<0.05),但均在正常范围内,无临床意义,其余指标与对照组比较差异均无统计学意义(P>0.05)。结论:苦荞醋饮无急性经口毒性和亚急性经口毒性,是安全、无毒的食品。Objective:To study whether buckwheat vinegar drink fermented from buckwheat for both medicine and food has acute oral toxicity and subacute oral toxicity,to provide animal test basis for safe food.Methods:In the acute oral toxicity test,the dose group was given 100 times of the recommended intake of the Tartary buckwheat vinegar drink by intragastric administration at a volume of 20 mL/kg,and the control group was given distilled water by gavage.The mice were observed for 14 days after gavage.To record the data on general observation,body weight changes,gross anatomical examination results,and organ coefficients data of the mice.In the 28⁃day oral toxicity test,the high,medium,and low dose groups were respectively given 100 times,50 times,25 times of the recommended intake of the Tartary buckwheat vinegar drink by intragastric administration at a volume of 10 mL/kg for 28 days continuously,and the control group was given distilled water by gavage.During the experiment,the general observation,body weight change,food intake,and food utilization rate of each group were recorded.At the end of the experiment,hematology examination,blood biochemical examination,gross anatomy examination,organ coefficient measurement,and histopathological examination were performed.Results:The mice did not appear symptoms of poisoning,and no death.Compared with the control group,there was no significant difference in the body weights and organ coefficients of major organs of the mice in the dose group(P>0.05).The rats did not appear symptoms of poisoning,and no death.The WBC of female rats in the low⁃dose group and the absolute weight of the spleen of male rats in the middle⁃dose group were significantly different from those in the control group(P<0.05),but they were all within the normal range,so they had no clinical significance.There was no significant difference in other indicators compared with the control group(P>0.05).Conclusion:This study proves that the Tartary buckwheat vinegar drink has no acute oral toxicity and subacu

关 键 词：苦荞醋饮 急性经口毒性试验 28 d经口毒性试验 安全性 药食两用物质 

分 类 号：R459.3[医药卫生—治疗学]