Design and construction of microbial cell factories based on systems biology  

作　　者：Wenlong Yan Zhibei Cao Mingzhu Ding Yingjin Yuan 

机构地区：[1]Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin,300072,China [2]Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin University,Tianjin,300072,China

出　　处：《Synthetic and Systems Biotechnology》2023年第1期176-185,共10页合成和系统生物技术（英文）

基　　金：by the National Key Research and Development Program of China (2019YFA0706900);National Natural Science Foundation of China (22278310).

摘　　要：Environmental sustainability is an increasingly important issue in industry.As an environmentally friendly and sustainable way,constructing microbial cell factories to produce all kinds of valuable products has attracted more and more attention.In the process of constructing microbial cell factories,systems biology plays a crucial role.This review summarizes the recent applications of systems biology in the design and construction of microbial cell factories from four perspectives,including functional genes/enzymes discovery,bottleneck pathways identification,strains tolerance improvement and design and construction of synthetic microbial consortia.Systems biology tools can be employed to identify functional genes/enzymes involved in the biosynthetic pathways of products.These discovered genes are introduced into appropriate chassis strains to build engineering microorganisms capable of producing products.Subsequently,systems biology tools are used to identify bottleneck pathways,improve strains tolerance and guide design and construction of synthetic microbial consortia,resulting in increasing the yield of engineered strains and constructing microbial cell factories successfully.

关 键 词：Microbial cell factories Systems biology Functional genes/enzymes discovery Bottleneck pathways Strains tolerance Synthetic microbial consortia 

分 类 号：F426[经济管理—产业经济]