液相色谱法检测鸡肉中芬苯达唑及其代谢物残留  

Determination of Fenbendazole and its metabolites in chicken by HPLC

作　　者：赵欣然 齐文婷 张伟 Zhao Xinran;Qi Wenting;Zhang Wei(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300450,China)

机构地区：[1]天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津300392 [2]天津药物研究院,天津300450

出　　处：《天津农学院学报》2023年第1期51-54,共4页Journal of Tianjin Agricultural University

基　　金：大学生创新训练项目(201910061138)。

摘　　要：建立鸡肉中芬苯达唑及其代谢物芬苯达唑砜和奥芬达唑残留的高效液相色谱检测方法。鸡肉组织样品捣碎后,加入无水硫酸钠,再用含有二甲基亚砜的乙酸乙酯提取,正己烷去脂,C18柱净化,氮气吹干,后加0.5 mL流动相(乙腈∶5 mol/L乙酸铵水溶液=1∶1)溶解。以乙腈和5 mol/L醋酸铵为流动相,梯度洗脱,流速为0.8 mL/min,上样体积为20μL,柱温为20℃,在波长为294 nm处检测。结果在0.02~0.50μg/mL范围内呈线性,R^(2)>0.999,在1~25μg/kg范围内,3种物质的平均回收率为77.9~98.6%,日内变异系数为3.10~6.81%(n=3),日间变异系数为1.19~7.74%(n=3)。该方法简单、灵敏、高效,适合于基层检测动物组织中芬苯达唑及其代谢物残留。A simple and sensitive liquid chromatography method that allows for the determination of Fenbendazole and its metabolites(Fenbendazole Sulfone and Offendazole)in chicken was established.The chicken tissue samples were mashed,then added with anhydrous sodium sulfate,and then extracted with ethyl acetate containing dimethyl sulfoxide.The extract was defatted with hexane,and then purified by C18 solid phase cartridge.The eluent was dried with nitrogen,and then dissolved with 0.5 mL mobile phase(acetonitrile∶5 mol/L ammonium acetate=1∶1).The final extracts were analysed by HPLC.The mobile phase was acetonitrile and 5 mol/L ammonium acetate at a flow rate of 0.8 mL/min.The sample volume was 20μL,the column temperature was room temperature,and the detection wavelength was 294 nm.The linear range was 0.02-0.50μg/mL,R^(2)>0.999.In the range of 1-25μg/kg,the average recoveries of the three materials were 77.9-98.6%,the intra day coefficient of variation was 3.10-6.81%(n=3),and the inter day coefficient of variation was 1.19-7.74%(n=3).The method is simple,sensitive,efficient,and suitable for the detection of fenbendazole and its metabolites in animal tissues.

关 键 词：芬苯达唑 芬苯达唑砜 奥芬达唑 液相色谱 残留检测 

分 类 号：S859.84[农业科学—临床兽医学]