BS-400全自动分枝杆菌液体培养系统对分枝杆菌培养的效果评价  

Evaluation of the effect of BS-400 Automatic Mycobacterium Liquid Culture System on Mycobacterium culture

作　　者：岳永宁 范大鹏 商学钗 李浩 李奂谕 周锦阳 蔡龙 Yue Yongning;Fan Dapeng;Shang Xuechai;Li Hao;Li Huanyu;Zhou Jinyang;Cai Long(Clinical Laboratory Center,Hangzhou Thoracic Hospital Affiliated to Zhejiang University School of Medicine,Hangzhou 310004,China)

机构地区：[1]浙江大学医学院附属杭州市胸科医院临床检验实验中心,杭州310004

出　　处：《中国防痨杂志》2023年第5期483-486,共4页Chinese Journal of Antituberculosis

基　　金：浙江省医药卫生科技计划项目(2023KY195)。

摘　　要：目的:评价BS-400全自动分枝杆菌液体培养系统(简称“BS-400培养系统”)在临床标本中培养分枝杆菌的效果。方法:搜集2022年3—5月在浙江大学附属杭州市胸科医院就诊的疑似结核病患者标本427份用于本研究,标本包括除血液和粪便外的331份呼吸道标本(包括痰液和呼吸道灌洗液)和96份非呼吸道标本(包括脑脊液、组织、分泌物、尿液、骨髓和坏死物等)。所有标本同时使用BACTEC MGIT 960液体培养系统(简称“MGIT 960培养系统”)和BS-400培养系统进行培养,培养阳性标本进行细菌分离和菌种鉴定。比较两种培养系统对不同类型标本分枝杆菌培养阳性率、检测时间、污染率及成本差异,并以MGIT 960培养系统培养结果为参照标准,评价BS-400培养系统对不同类型标本分枝杆菌感染的检测效能。结果:BS-400培养系统对呼吸道标本的培养阳性率为32.02%(106/331),污染率为5.14%(17/331);对非呼吸道标本的培养阳性率为17.70%(17/96),污染率为1.04%(1/96),与MGIT 960培养系统[分别为32.93%(109/331)和5.44%(18/331),15.63%(15/96)和2.08%(2/96)]相比,差异均无统计学意义(χ^(2)值分别为0.062、0.030、0.150、0.339,P值分别为0.803、0.862、0.699、1.000)。BS-400培养系统检测时间为12.31(7.89,19.56)d,MGIT 960培养系统检测时间为9.79(7.00,16.58)d,差异无统计学意义(Z=-1.895,P=0.058)。以MGIT 960培养系统培养结果为参照标准,BS-400培养系统检测呼吸道标本分枝杆菌感染的敏感度为93.58%(102/109)、特异度为98.20%(218/222),Kappa值为0.924;检测非呼吸道标本分枝杆菌感染的敏感度为100.00(15/15)、特异度为97.53%(79/81),Kappa值为0.925。BS-400培养系统检测成本[(55±10)元/份]约为MGIT 960培养系统检测成本[(75±10)元/份]的70%。结论:BS-400培养系统对疑似结核病患者临床标本中分枝杆菌的检测效能较高,具有较好的应用价值。Objective:To evaluate the effectiveness of the BS-400 Automatic Mycobacterium Liquid Culture System(BS-400 Culture System)in cultivating Mycobacterium in clinical specimens.Methods:A total of 427 specimens of suspected tuberculosis patients who visited Hangzhou Thoracic Hospital Affiliated to Zhejiang University School of Medicine from March to May 2022 were collected.The specimens included 331 respiratory tract specimens(including sputum and respiratory lavage fluid)and 96 non-respiratory tract specimens(including cerebrospinal fluid,tissues,secretions,urine,bone marrow,necrosis,etc.),except blood and feces.All specimens were simultaneously cultured using the BACTEC MGIT 960 liquid culture system(MGIT 960 culture system)and the BS-400 culture system.Bacterial isolation and species identification would be carried out on samples with positive culture results.The differences in the positive rate,detection time,contamination rate,and cost of two culture systems for different types of Mycobacterium specimens were compared,and the detection efficiency of the BS-400 culture system for Mycobacterium infections in different types of specimens was evaluated using the culture results of the MGIT 960 culture system as a reference standard.Results:The positive rate of BS-400 culture system for respiratory tract specimens was 32.02%(106/331),and the contamination rate was 5.14%(17/331);the positive rate of culture for non-respiratory tract specimens was 17.70%(17/96),and the contamination rate was 1.04%(1/96),compared with those of MGIT 960 culture system(32.93%(109/331)and 5.44%(18/331),15.63%(15/96)and 2.08%(2/96),respectively),the difference was not statistically significant(χ^(2) values were 0.062,0.030,0.150,and 0.339,P values were 0.803,0.862,0.699,and 1.000,respectively).The detection time was 12.31(7.89,19.56)days of the BS-400 culture system,and was 9.79(7.00,16.58)days of the MGIT 960 culture system,with no statistically significant difference(Z=-1.895,P=0.058).Using the culture results of MGIT 960 culture system a

关 键 词：分枝杆菌属 感染 培养技术 评价研究 

分 类 号：R378.91[医药卫生—病原生物学]