花生种子在低温胁迫下的萌发抑制生理研究  

Study on Germination Inhibition Physiology of Peanut Seeds under Low Temperature Stress

作　　者：王艺喆 苏良辰 WANG Yizhe;SU Liangchen(Zhuhai Compus of Zunyi Medical University,Zhuhai Guangdong 519041,China)

机构地区：[1]遵义医科大学珠海校区,广东珠海519041

出　　处：《种子》2023年第2期28-33,共6页Seed

基　　金：国家自然科学基金地区项目(31860310)。

摘　　要：为探究花生种子在低温胁迫时萌发抑制的内在机理,测定了低温条件下花生种子在萌发早期不同阶段的生理生化特征及萌发相关植物激素合成相关基因的表达变化。结果表明,与对照组相比,花生种胚中的脯氨酸和丙二醛含量在低温处理的各个时间点均有不同程度的下降,而超氧化物歧化酶和过氧化物酶活性在低温胁迫下呈上调表达的变化趋势。同时,各处理组可溶性蛋白和可溶性糖含量在早期萌发均呈下降趋势,但在低温处理组中下降的幅度较正常处理组显著。表明低温条件降低了种子萌发过程中的代谢速率,并通过提高细胞抗氧化酶的含量增强种子的生存能力。低温条件下,种子中的萌发促进类植物激素合成基因(AhYUC1、AhGA20OX1和AhIPT5)的表达虽然较对照组明显上调,但萌发抑制类植物激素合成基因(AhACS1和AhNCED1)表达量增加较对照组更加显著,说明低温胁迫条件下花生种子主要通过提高脱落酸和乙烯的含量抑制种子萌发。In order to explore the internal mechanism of inhibiting germination of peanut seeds under low temperature stress,the physiological and biochemical characteristics of peanut seeds at different stages of germination and the expression changes of germination-related plant hormone synthesis-related genes were measured.The results showed that compared with the control group,the contents of proline and malondialdehyde in peanut seed embryos decreased at different time points,while the activities of superoxide dismutase and peroxidase showed a trend of up-regulation under low temperature stress.At the same time,soluble protein and soluble sugar contents showed a downward trend in each group of early germination,but the decrease amplitude in the low-temperature treatment group was significantly lower than that in the normal treatment group.It indicated that low temperature reduced the metabolic rate of seeds during germination and enhanced the viability of seeds by increasing the content of antioxidant enzymes.At low temperature,the expressions of germination-promoting hormone-like genes(AhYUC1,AhGA20OX1 and AhIPT5) in seeds were significantly up-regulated compared with those in the control group,but the expressions of germination-inhibiting hormone-like genes AhACS1 and AhNCED1 increased more significantly than those in the control group.The results indicated that peanut seed germination was inhibited mainly by increasing abscisic acid and ethylene content under low temperature stress.

关 键 词：花生 低温胁迫 内源激素 萌发 抗氧化酶 

分 类 号：S565.2[农业科学—作物学]