红棕杜鹃(原变种)叶中一个新的杜鹃色烯杂萜及其抗凝血活性评价  

A new Rhododenderon chromene meroterpenoid from leaves of Rhododendron rubiginosum Franch.var.rubiginosum and its anticoagulant activity

作　　者：杨勇勋[1] 颜瑜[2] 黄海燕 YANG Yong-xun;YAN Yu;HUANG Hai-yan(School of Animal Sciences,Xichang University;Xichang University Library;School of Sciences,Xichang University,Xichang 615000,China)

机构地区：[1]西昌学院动物科学学院 [2]西昌学院图书馆 [3]西昌学院理学院,西昌615000

出　　处：《天然产物研究与开发》2023年第4期607-611,共5页Natural Product Research and Development

基　　金：国家自然科学基金(81860758);凉山州科学与技术带头人培养资金项目(2022)。

摘　　要：采用硅胶、MCI Gel、Sephadex LH-20、ODS等柱色谱,结合半制备液相色谱,从红棕杜鹃(原变种)叶乙醇提取物的石油醚部位分离纯化得到一对双键位置异构的杜鹃色烯杂萜化合物:isoconfluentin(1)和(+)-confluentin(2),其中化合物1为新化合物。运用现代波谱学方法(1D和2D NMR、UV、IR、MS等)对所分离得到的化合物进行了结构鉴定,而且采用色烷/色烯螺旋规则(chromane/chromene helicity rule)鉴定它们的绝对构型均为2 S构型。测定化合物1和2的体外凝血酶原时间,发现它们均具有有效的抗凝血作用。Two Rhododenderon chromene meroterpenoids,isoconfluentin(1)and(+)-confluentin(2),were isolated from the petroleum ether extract of ethanol fraction of the leaves of Rhododendron rubiginosum Franch.var.rubiginosum by using various chromatographic methods,including MCI Gel,sephadex LH-20,ODS,silica gel and semi-preparative HPLC.Their planar structures were identified by spectrographic methods,such as NMR,UV,IR,MS,and the absolute configuration of C-2 of 1 and 2 was determined to be the S configuration by the chromane/chromene helicity rule.Compound 1 is a new compound,and compounds 1 and 2 showed effective anticoagulant effect via prothrombin time assays in vitro.

关 键 词：红棕杜鹃(原变种) 杜鹃色烯杂萜 抗凝血作用 

分 类 号：R284[医药卫生—中药学]