民族药四数九里香的化学成分及其细胞毒活性研究(Ⅲ)  

Compounds from Murraya tetramera Huang and their cytotoxic activity(Ⅲ)

作　　者：周永福 姚如杰[1] 黎学明 杨成威[1] ZHOU Yong-fu;YAO Ru-jie;LI Xue-ming;YANG Cheng-wei(Chongqing Zi-Guang Chemical Co.,Ltd.,Chongqing 401121,China;College of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400044,China;Chongqing Industry Polytechnic College,Chongqing 401120,China)

机构地区：[1]重庆紫光化工股份有限公司,重庆401121 [2]重庆大学化学化工学院,重庆400044 [3]重庆工业职业技术学院,重庆401120

出　　处：《天然产物研究与开发》2023年第4期622-629,共8页Natural Product Research and Development

基　　金：重庆紫光化工股份有限公司博士后项目(KQY20220530)。

摘　　要：阐释民族药四数九里香Murraya tetramera Huang的药效物质基础。本文采用现代色谱分离技术从其醇提取物的乙酸乙酯萃取部位中分离和纯化得15个化合物,采用核磁共振波谱技术和文献数据比对方法鉴定了化合物结构,分别为luteolin-7-O-glucoside(1)、3,4-dihydroxy benzeneacrylic acid(2)、2-hydroxylcinnamie acid(3)、3,8-dihydroxy-4-methoxy-coumarin(4)、2-methyl-6-hydroxychromone(5)、chlorogenic acid(6)、methyl caffeate(7)、furo[3,2-α]carbazole(8)、(E)-2-((4-hydroxyphenyl)diazenyl)phenol(9)、N-(2,6-dimethylphenyl)-N′-(4′-nitrophenyl)urea(10)、九里香碱(11)、phorbazole C(12)、3-羟苯基喹唑酮(13)、N-反式阿魏酰基-3-甲基多巴胺(14)、6-丙酮基-5,6-二氢血根碱(15)。15个化合物均为首次从该植物中分离到;采用MTS法分析了15个化合物的体外抑制肿瘤细胞增殖作用,结果表明15个化合物对5株肿瘤细胞的体外生长具有不同程度的抑制作用,其中化合物1、8~15对人肿瘤细胞株HL-60、A-549、SMMC-7721、SW-480、MCF-7具有较好的抑制增殖作用。In order to study the pharmacodynamic material basis of the Murraya tetramera Huang;in this paper,fifteen compounds were isolated and purified from the ethyl acetate extract of its alcohol extract by modern chromatographic separation technology.And Identified the structure of the compounds by NMR spectroscopy,which were luteolin-7-O-glucoside(1),3,4-dihydroxy benzeneacrylic acid(2),2-hydroxylcinnamie acid(3),3,8-dihydroxy-4-methoxy-coumarin(4),2-methyl-6-hydroxychromone(5),chlorogenic acid(6),methyl caffeate(7),furo[3,2-α]carbazole(8),(E)-2-((4-hydroxyphenyl)diazenyl)phenol(9),N-(2,6-dimethylphenyl)-N′-(4′-nitrophenyl)urea(10),murrayanine(11),phorbazole C(12),3-(2’-hydroxyphenyl)-4(3 H)-quinazolidone(13),N-trans-feruloyl-3-methyldopamine(14),6-acetone-5,6-dihydrosanguinarine(15).All compounds are isolated from this plant for the first time.The tumor inhibitory and proliferation effects of 15 compounds were analyzed by the MTS method.The inhibitive effects of the compounds on the growth of five tumor cells in vitro were studied.Especially,the compound 1,8-15 have good growth inhibitory effect on human tumor cell lines HL-60,A-549,SMMC-7721,SW-480 and MCF-7.

关 键 词：民族药 四数九里香 化学成分 抑制细胞增殖作用 

分 类 号：R284.2[医药卫生—中药学]