南极钩虾Gammarussp.肠道共附生菌的分离及StreptomycesxiamenensisYF008的化学成分研究  

Isolation of intestinal symbiotic or epiphytic microbes from Gammarus sp.and chemical characterization of Streptomyces xiamenensis YF008

作　　者：代影 卢静容 周美宏 杨帆[2] 林厚文[2] DAI Ying;LU Jingrong;ZHOU Meihong;YANG Fan;LIN Houwen(College of Food Science and Technology,Shanghai Ocean University,Shanghai201306,China;Department of Pharmacy,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai200127,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海交通大学医学院附属仁济医院药学部,上海200127

出　　处：《海洋渔业》2023年第2期129-139,共11页Marine Fisheries

基　　金：国家自然科学基金联合基金项目(U2106227);上海市人才发展资金;上海市高水平地方高校建设创新团队项目。

摘　　要：南极极端生境中蕴含着发掘特殊生物活性天然产物的巨大潜力。对采自南极海域水深6000m海区钩虾(Gammarussp.)的肠道共附生微生物进行分离,并对一株有价值菌株的代谢产物进行研究。采用稀释涂布法进行菌株分离,利用形态学观察及16SrRNA测序进行菌株种属鉴定;综合运用薄层色谱、正相硅胶柱色谱、凝胶柱色谱、反相ODS柱色谱和半制备高效液相(HPLC)等色谱手段分离纯化得到单体化合物,采用波谱学和质谱技术鉴定其化学结构。从钩虾中分离得到17株菌,并将其从分类学角度归属于9个属,其中细菌11株,放线菌6株。从放线菌StreptomycesxiamenensisYF008的发酵产物中分离得到8个单体化合物,分别鉴定为:1(10)E,5E-germacradiene-2,11-diol(1),N-[2-(4-hydroxyphenyl)]ethylacetamide(2),环(L-苯丙氨酸-甘氨酸)(3),环(L-苯丙氨酸-L-丙氨酸)(4),环(D-苯丙氨酸-L-异亮氨酸)(5),环(L-羟脯氨酸-L-苯丙氨酸)(6),环(D-亮氨酸-L-脯氨酸)(7),环(L-苯丙氨酸-L-脯氨酸)(8)。化合物1、3~6系首次从该菌中分离得到,且化合物1为特殊的十元环germacrane型倍半萜类化合物。The extreme habitats of the Antarctica have great potential to discover special biologicallyactive natural products.Polar microorganisms have formed survival mechanisms and biosynthetic pathways different from terrestrial strains to adapt to the unique environment of polar regions.Polar actinomycetes have metabolized a series of novel microbial natural products with interesting structures.Polar microbes have greater potential for exploitation as sources of novel active secondary metabolites than terrestrial microbes.The microbiota within the NorthSouth polar circle,a powerful producer of novel natural products,has become the target of scholars in natural product research.Gammarid(Gammarus sp.)is a kind of crustaceans belonging order Amphipoda,suborder Gammaridea,family Gammaridae that can live in the Antarctic deep sea.At present,there are few reports on the intestinal symbiotic or epiphytic microbes of Gammarus sp.,and there is large space for innovative development on the isolation of intestinal intestinal symbiotic or epiphytic microbes of Gammarus sp.and the study of their secondary metabolites.This study is aim to discover and identify the intestinal symbiotic or epiphytic microbes from Gammarus sp.6000 m deepth in the Antarctic sea,and the chemical structural diversity of metabolites of a valuable actinomycete strain obtained from Gammarus sp.were studied.The strain was isolated by gradient dilution coating method with inverted cultivation under 28℃for 221 days.After the growth of colony,the single colony was obtained by lineation purification.The isolation medium was R2A,SC,M7,SIM2,F,2216E and M10 solid medium,and the purification medium was Gao’s No.1,SFM and LB solid medium.The isolated strains were cultured on the fresh medium plates and identified by morphological observation and 16S rRNA sequencing.Genomic DNA of the strains was extracted,PCR amplified with bacterial universal primers 27F and 1492R,and the species genera of the strains were identified by 16S rRNA sequencing after agarose

关 键 词：南极钩虾 放线菌 Streptomyces xiamenensis 代谢产物 分离鉴定 

分 类 号：S917.1[农业科学—水产科学]