盐度胁迫对彩虹明樱蛤存活、抗氧化酶和Na^(+)/K^(+)-ATP酶活性的影响  

Effects of salinity stress on survival,antioxidant enzymes and Na+/K+-ATPase activities of Moerella iridescens

作　　者：裴瑞华 郑梓瑶 张诺 刘其根[1,2] 陈丽平 胡忠军[1,2] PEI Ruihua;ZHENG Ziyao;ZHANG Nuo;LIU Qigen;CHEN Liping;HU Zhongjun(Key Laboratory of Freshwater Aquatic Genetic Resources of Ministry of Agriculture andRural Affairs,Shanghai Ocean University,Shanghai201306,China;Centre for Research onEnvironmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture andRural Affairs,Shanghai Ocean University,Shanghai201306,China)

机构地区：[1]上海海洋大学,农业农村部淡水水产种质资源重点实验室,上海201306 [2]上海海洋大学,农业农村部鱼类营养与环境生态研究中心,上海201306

出　　处：《海洋渔业》2023年第2期162-171,共10页Marine Fisheries

基　　金：国家重点研发计划项目(2020YFD0900504,2017YFC0506003)。

摘　　要：为探究彩虹明樱蛤(Moerella iribescens)对盐度胁迫的适应能力和调节机制,对其进行急性盐度胁迫实验,在盐度5~50之间设定10个盐度梯度(5,6,8,11,14,18,23,30,39,50),观察死亡情况。结果显示,盐度胁迫96 h后,盐度50组死亡率为100%,盐度39、30、23、6、5死亡率分别为43.3%、6.7%、3.3%、1.7%、8.3%,而盐度8~18之间的4个组中未发现死亡个体。根据急性胁迫实验结果设置5个盐度梯度(6、12、18、24、30),测定0、24、48、72、96 h鳃和消化腺超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和Na^(+)/K^(+)-ATP酶活性变化。结果显示,在同一盐度胁迫下,彩虹明樱蛤鳃和消化腺的SOD、CAT、GSH-Px和Na^(+)/K^(+)-ATP酶活性随时间的变化呈先上升后下降并趋于稳定的变化趋势,不同盐度组SOD、CAT和GSH-Px活性在24 h或48 h达到高峰,不同盐度组Na^(+)/K^(+)-ATP活性在48 h或72 h达到高峰。研究表明,彩虹明樱蛤的适宜盐度为8~18,3种抗氧化酶对盐度胁迫响应时间短于Na^(+)/K^(+)-ATP酶。研究从生存适应和应激相关酶活性角度探讨了彩虹明樱蛤对盐度的响应,可为其养殖和保育提供科学依据。Salinity is an important ecological factor relative to the survival,reproduction,growth,and development of aquatic life forms through influencing the energy consumption in the process of osmotic regulation and antioxidant enzyme activity of animal’s organism.The salinity of estuary changes drastically,which will exert a great physiological stress on shellfish with limited migration ability.The sodium-potassium ATP enzyme(Na^(+)/K^(+)-ATPase)is an important dynamic component of sodium-potassium pump,and its activity is often used as an important indicator of osmotic regulation ability in animals.Free radical scavenger enzymes such as superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)are important members of the antioxidant enzyme system and are effective indicators to assess the effect of the environment on life form.Rainbow clam(Moerella iridescens)is an intertidal shellfish with high economical value.Historically,it was a dominant species in intertidal zone of the Yangtze River Estuary.Moerella iridescens is required to be protected urgently for its sharp decline in population size and drastic contraction in distribution area under multiple environmental stresses.However,the salinity in estuary fluctuates largely.Shellfishes inhabiting in estuaries often regulate body functions to adapt to environmental changes.Therefore,it has important scientific significance and application value for protection and rearing of Moerella iridescens to reveal its adaptation to salinity variation and its regulation mechanism.In present study,acute stress experiments were carried out to explore the adaptability to salinity and regulation mechanism of Moerella iridescens,in which the mortality rate and activities of Na^(+)/K^(+)-ATPase and three antioxidant enzymes were measured under different salinity gradients and exposure durations.Moerella iridescens was purchased in the aquatic product market of Luchao Port,Pudong New Area,Shanghai.Healthy and vigorous experimental clams,with(17.37±0.36)mm of average

关 键 词：彩虹明樱蛤 盐度 死亡率 抗氧化酶 Na^(+)/K^(+)-ATP酶 

分 类 号：S917.4[农业科学—水产科学]