丁香酚和MS-222对松江鲈的麻醉效果  

Anesthetic effects of eugenol and MS-222 on Trachidermus fasciatus

作　　者：胡望娇 王翠华 冯广朋[1,2] 庄平[2] 郑跃平[3] 季强 HU Wangjiao;WANG Cuihua;FENG Guangpeng;ZHUANG Ping;ZHENG Yueping;JI Qiang(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Shanghai YangtzeEstuary Fishery Resources Proliferation and Ecological Restoration Engineering Technology Research Center,East ChinaSea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai200090,China;Shanghai AquaticWildlife Conservation and Research Center,Shanghai202162,China;Shanghai Songjiang District AquaticTechnology Promotion Station,Shanghai201669,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院东海水产研究所,上海长江口渔业资源增殖和生态修复工程技术研究中心,上海200090 [3]上海市水生野生动植物保护研究中心,上海202162 [4]上海市松江区水产技术推广站,上海201699

出　　处：《海洋渔业》2023年第2期181-190,共10页Marine Fisheries

基　　金：国家重点研发计划重点专项(2020YFD0900805);农业农村部物种资源保护专项(17210163);上海市水生生物(濒危珍稀物种)增殖放流效果评估项目(2021)。

摘　　要：采用静水试验方法,研究了不同质量浓度的两种常用渔用麻醉剂丁香酚(5、10、20、40、80、120 mg·L^(-1))和MS-222(40、50、60、75、100、200 mg·L^(-1)对体质量为(13.78±3.15)g的松江鲈(Trachidermus fasciatus)的麻醉效果。结果表明:(1)随着两种麻醉剂浓度的升高,松江鲈进入麻醉状态的时间逐渐缩短,复苏时间不断延长;(2)丁香酚与MS-222的最适麻醉质量浓度分别为40~80 mg·L^(-1)和60 mg·L^(-1);(3)在低浓度麻醉剂处理下,10 mg·L^(-1)丁香酚组中松江鲈的耗氧率显著低于对照组(P<0.05),表明10 mg·L^(-1)丁香酚可能是松江鲈的适宜运输浓度;(4)对比成活率、进入麻醉状态的时间和复苏时间等指标,丁香酚更适合作为松江鲈的麻醉剂,在实际操作中,建议使用40~80 mg·L^(-1)丁香酚进行松江鲈的麻醉。The main purpose of this study is to explore the anesthetic effect of eugenol and MS-222 on Trachidermus fasciatus.T.fasciatus is a kind of small benthic,carnivorous,wide temperature and wide salinity migratory fish,whose resources have collapsed in recent years since the 1980s.It was listed as an endangered species in China Red Data Book of Endangered Animals in 1998.Enhancement and release are considered to be important and effective measures for ecological restoration and resource conservation.As the secondary protected aquatic wildlife in China,a lot of T.fasciatus enhancement and releasing has been carried out by relevant departments in recent years.However,some activities such as marking and transportation may cause stress response and damage to the fish,which in turn may reduce the survival rate of T.fasciatus in the process of enhancement and releasing.Anaesthesia is one of the most important means to reduce fish stress and improve survival rates in the releasing.The two most commonly used fish anaesthetics in China are eugenol and MS-222,which are generally considered to be safe and effective anaesthetics in aquatic animals,and they have been widely used throughout the world.At present,eugenol and MS-222 have been used for anesthesia in numerous fish species at home and abroad,but no relevant studies on their anesthetic effects on T.fasciatus have been reported.This study was conducted to compare the anaesthetic effects of different mass concentrations of eugenol and MS-222 on T.fasciatus to provide scientific reference for aquaculture production and releasing of T.fasciatus.Three different anesthesia experiments were designed by immersion for this experiment.The specific experimental approaches were as follows:1)Record the final anesthesia degree and recovery status of the fish under different concentrations of eugenol and MS-222 anesthesia;2)Observe and record the time when T.fasciatus entered each anesthesia stage and the time of the recovery stage during the recovery process under two different anaes

关 键 词：麻醉 丁香酚 MS-222 松江鲈 耗氧率 

分 类 号：S917[农业科学—水产科学]