大白菜Ogura胞质雄性不育恢复材料的创制与检测  

Development and detection of fertility- restored BC_(3) progenies in Ogura CMS Chinese cabbage

作　　者：魏小春[1] 原玉香[1] 赵艳艳[1] 杨双娟[1] 苏贺楠 王志勇[1] 王晓青 冉冉 李林 牛刘静 张晓伟[1] WEI Xiaochun;YUAN Yuxiang;ZHAO Yanyan;YANG Shuangjuan;SU He'nan;WANG Zhiyong;WANG Xiaoqing;RAN Ran;LI Lin;NIU Liujing;ZHANG Xiaowei(Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省农业科学院园艺研究所,郑州450002

出　　处：《中国瓜菜》2023年第4期37-46,共10页China Cucurbits And Vegetables

基　　金：河南省科技攻关计划项目(212102110124);河南省重点研发专项(221111110100);河南省农业科学院科技创新团队(2022TD06)。

摘　　要：针对白菜类蔬菜(Brassica rapa L.)中缺乏Ogura胞质雄性不育育性恢复材料的问题,利用远缘杂交技术将恢复基因Rfo从甘蓝型油菜导入大白菜中,利用种间杂交种不断与不育系进行回交,已分别获得BC_(1)、BC_(2)和BC_(3)代育性恢复材料。同时,利用形态学、细胞遗传学、分子生物学手段对所创制的材料进行检测,利用恢复基因Rfo特异性引物扩增,最后筛选获得了一株育性稳定、形态特征接近大白菜、染色体数目为20的BC_(3)单株。利用所创制BC_(3)单株进行Ogura雄性不育大白菜商品杂交种恢复测试,结合表型及恢复基因Rfo、不育基因orf138特异性引物检测,结果表明,所创制恢复系恢复基因有效。Aiming at the lack of restorer of-fertility germplasm resources in Ogura cytoplasmic male sterility(Ogura CMS)Brassica rapa,the Rfo(restorer of fertility)gene was introduced from Brassica napus to Chinese cabbage by distal hybridization,and BC_(1),BC_(2) and BC_(3) generation fertility recovery materials were obtained respectively.Through morphology,cytogenetic and molecular biological methods,meanwhile,combined with the PCR products with Rfo-specific primers,a single BC_(3) plant with stable fertility,similar morphological characteristics,with 20 chromosomes was obtained.The recovery test of commercial Ogura CMS hybrid Chinese cabbage was carried out using the BC_(3) Rfo gene created plant,combined with phenotype checking,Rfo and orf138 specific primer detection,which showed that the restorer of fertility gene of the created line was effective.

关 键 词：大白菜 Ogura胞质不育 恢复基因 材料创制 

分 类 号：S634.1[农业科学—蔬菜学]