枯萎病菌毒素粗提液对美洲南瓜幼苗生长及生理的影响  

Effects of crude toxin extract in the pathogen causing Cucurbita pepo Fusarium wilt on the growth and physiology of seedlings

作　　者：刘佳 柴改凤 郭风清 张树武 徐秉良 LIU Jia;CHAI Gaifeng;GUO Fengqing;ZHANG Shuwu;XU Bingliang(Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province/College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃省农作物病虫害生物防治工程实验室·甘肃农业大学植物保护学院,兰州730070

出　　处：《中国瓜菜》2023年第4期81-88,共8页China Cucurbits And Vegetables

基　　金：2021年甘肃省教育厅青年博士基金项目(2021QB-025);甘肃农业大学科技创新基金(GAU-XKJS-2018-156);国家自然科学基金(31460518)。

摘　　要：为了揭示美洲南瓜枯萎病菌产生的毒素粗提液对幼苗的影响,研究了病原菌产毒的条件以及不同浓度毒素粗提液对美洲南瓜幼苗生长及生理的影响。结果表明,美洲南瓜枯萎病菌在pH值=7的Czapek培养液中24 h连续光照、振荡培养15 d时,产毒效果最明显,对美洲南瓜种子胚根生长抑制率最高,毒素粗提液亦具有较强的热稳定性。80%的病原菌毒素粗提液,对美洲南瓜株高、茎粗和根长的生长抑制效果最好。处理11 d时,80%毒素粗提液处理下,美洲南瓜幼苗根系和叶片的细胞膜透性分别达到62.71%和62.31%;叶片MDA含量为对照的2.61倍,叶片PAL活性最高,为84.25 U·g^(-1),是对照处理PAL酶活性的2.09倍。研究结果可为揭示美洲南瓜枯萎病菌的致病机制提供一定的理论依据。In order to reveal the effect of crude toxin produced by pathogen causing Cucurbita pepo Fusarium wilt,the toxin-producing conditions of Fusarium oxysporum and the effects of different concentrations of toxin crude extract on the growth and physiology of Cucurbita pepo seedlings were studied.The results showed that:In the Czapek medium of pH=7,24 hours of continuous light and shaking culture for 15 days,the toxigenic effect was the most obvious,and the growth inhibition rate of the radicle of Cucurbita pepo seeds was the highest.The crude toxin extract has strong thermal stability.80%of the toxin crude extract had the greatest growth inhibitory effect on plant height,stem diameter and root length.Under the treatment of 80%crude toxin extract,the cell membrane permeability of Cucurbita pepo seedlings roots and leaves reached 62.71%and 62.31%on the 11th day of treatment,respectively;The content of MDA in leaves was 2.61 times that of the control,and the activity of PAL in leaves was the highest,which was 84.25 U·g^(-1),which was 2.09 times that of the control.The research results provided the theoretical basis for revealing the pathogenic mechanism of Cucurbita pepo Fusarium wilt.

关 键 词：美洲南瓜 枯萎病菌 毒素 生长 细胞膜透性 生理指标 

分 类 号：S642.6[农业科学—蔬菜学]