瓜实蝇成虫对不同杀虫剂相对敏感基线的建立  

The relative sensitivity baseline of Zeugodacus cucurbitae(Diptera: Tephritidae)adults to different insecticides

作　　者：陈黔 杨朗 黄立飞[2,3] 曹雪梅 张建民[1] 姜建军 CHEN Qian;YANG Lang;HUANG Lifei;CAO Xuemei;ZHANG Jianmin;JIANG Jianjun(College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China(Ministry of Agriculture and Rural Affairs),Nanning 530007,Guangxi,China;Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests/Plant Protection Research Institute,Guangxi Academy of Agricultural Science,Nanning 530007,Guangxi,China)

机构地区：[1]长江大学农学院,湖北荆州434025 [2]农业农村部华南果蔬绿色防控重点实验室,南宁530007 [3]广西作物病虫害生物学重点实验室·广西壮族自治区农业科学院植物保护研究所,南宁530007

出　　处：《中国瓜菜》2023年第4期106-112,共7页China Cucurbits And Vegetables

基　　金：国家自然科学基金(31960564);广西科技重大专项(AA20108002);广西农业科学院发展基金(2021JM72,2021YT068)。

摘　　要：为了监测瓜实蝇田间抗药性和筛选用于防治该害虫的化学药剂,采用点滴法和饲毒法,研究测定了17类30种杀虫剂对瓜实蝇成虫的毒力并建立了相对敏感基线,同时采用建立的相对敏感基线比较分析了点滴法和饲毒法对抗阿维菌素品系抗性水平测定结果的差异。结果表明,采用点滴法测得9类18种药剂LD50值在3.422~576.842 ng·头^(-1);采用饲毒法测得10类21种药剂LC50值在0.035~60.542 mg·L^(^(-1))。2种方法测得的不同药剂对瓜实蝇毒力高低排序较一致,3种微生物源农药甲维盐、多杀霉素、阿维菌素和有机磷类乐果对瓜实蝇均表现出较高的毒力,菊酯类、有机磷类和烟碱类等药剂次之,鱼藤酮毒力最低。点滴法和饲毒法测得的瓜实蝇抗阿维菌素品系的抗性倍数分别为18.22倍和17.37倍,较为接近。综上所述,采用点滴法和饲毒法分别建立了瓜实蝇成虫对18种和21种杀虫剂的相对敏感基线,为瓜实蝇的抗药性监测和化学防治提供了科学依据。In order to monitor the resistance of Zeugodacus cucurbitae(Diptera:Tephritidae)in the field and screen chemical agents for pest control,the toxicity of the adults of Z.cucurbitae to thirty insecticides belonging to seventeen pesticide categories were determined using topical and feeding applications,the relative sensitivity baseline of melon fly to different insecticides were established.And,the resistance level of an abamectin-resistant strain(RS)was investigated by two bioassay methods.The results showed that the LD50 values of the eighteen insecticides to melon fly were 3.422-576.842 ng·fly^(-1) by topical application,LC50 values of the twenty-one insecticides were 0.035-60.542 mg·L^(^(-1)) by feeding application.The toxicity ranks of two application were similar,three microbial insecticides emamectin benzoate,spinosad,abamectin and organophosphorus insecticide dimethoate,showed high toxicity to Z.cucurbitae,followed by pyrethroids,organophosphorus and nicotinoids insecticides,and rotenone with the lowest toxicity.The resistance ratio(RR)of abamectin-resistant strain was 18.22 folds based on LD50,while it was 17.37 folds based on LC50,and the RR of two applications was similar.In conclusion,the relative sensitivity baseline of Z.cucurbitae adults to eighteen and twenty-one insecticides have been established using topical and feeding applications respectively,providing scientific basis for the resistance monitoring and chemical control of Z.cucurbitae.sensitivity baseline.

关 键 词：瓜实蝇 杀虫剂 敏感基线 抗药性 

分 类 号：S482.3[农业科学—农药学]