基于黄褐棉导入系群体定位抗黄萎病QTL  

Mapping QTL for Verticillium wilt resistance based on the population of Gossypium mustelinum introgression lines

作　　者：常鑫燚 李轩照 唐秉晖 潘振远 吴元龙 沈超 努日曼古丽·艾尼 林忠旭 尤春源[2,4] 聂新辉 Chang Xinyi;Li Xuanzhao;Tang Binghui;Pan Zhenyuan;Wu Yuanlong;Shen Chao;Nurimanguli Aini;Lin Zhongxu;You Chunyuan;Nie Xinhui(Agricultural College of Shihezi University/The Key Laboratory of Oasis Eco-agriculture,Xinjiang Production and Construction Corps,Shihezi,Xinjiang 832003,China;Cotton Research Institute,Shihezi Academy of Agriculture Science,Shihezi,Xinjiang 832011,China;College of Biological and Food Engineering,Guangdong University of Petrochemical Technology,Maoming,Guangdong 525000,China;National Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,新疆石河子832003 [2]石河子农业科学院棉花研究所,新疆石河子832011 [3]广东石油化工学院生物与食品工程学院,广东茂名525000 [4]华中农业大学植物科学技术学院/作物遗传改良国家重点实验室,武汉430070

出　　处：《棉花学报》2023年第1期29-38,共10页Cotton Science

基　　金：新疆生产建设兵团中青年领军人才项目(2020CB017);新疆生产建设兵团科技创新人才计划(科技特派员)(S2019CB1877);新疆生产建设兵团科技创新人才计划(强青)(2021CB028);石河子市农业科技攻关项目(2021NY01);石河子市财政科技计划项目(2022NY01)。

摘　　要：【目的】黄萎病(Verticillium wilt)是棉花生产中的最主要病害,且棉花黄萎病的致病机理尚不清楚。通过构建黄褐棉导入系群体定位棉花黄萎病抗性相关的数量性状位点(quantitative trait loci,QTL),为抗黄萎病分子标记开发和辅助育种提供参考。【方法】以陆地棉(Gossypium hirsutum)B0011为轮回亲本、黄褐棉(G.mustelinum)为供体亲本,构建有71个株系的BC_(5)S_(5)群体。利用2 839个单核苷酸多态性(single nucleotide polymorphism,SNP)标记结合表型值进行黄萎病抗性相关QTL定位。【结果】共检测到15个与黄萎病抗性相关的QTL,可解释4.21%~26.77%的表型变异。加性效应分析表明:其中6个QTL的有利等位基因来源于黄褐棉,9个QTL有利等位基因来自B0011。同时,qVW-A01-1、q VW-A02-2和qVW-A07-2在2个及以上环境中被检测到,表型变异解释率分别为15.56%~16.56%、11.95%~24.62%和13.22%~16.73%。利用BC5S5群体黄萎病抗性的最佳线性无偏预测值(best linear unbiased prediction,BLUP)进行QTL定位,共检测到5个QTL,其中qVW-A01-1B、q VW-A02-1B分别与加性效应分析稳定检测到的qVW-A01-1、qVW-A02-2物理位置一致,分别解释23.67%和17.90%的表型变异。【结论】本研究发现2个稳定的QTL即qVW-A01-1和qVW-A02-2,可为抗黄萎病分子标记辅助选择育种及候选基因功能鉴定奠定基础。[Objective]Verticillium wilt(VW)is one of the most significant diseases affecting cotton production,and the pathogenesis of VW in cotton is still unclear.By constructing a Gossypium mustelinum introgression population,this study aims to locate quantitative trait loci(QTL)related to VW resistance and provides a reference for the development of molecular markers for VW resistance and assisted breeding.[Method]A BC_(5)S_(5) population containing 71 introgression lines was constructed using upland cotton(G.hirsutum)B0011 as the recurrent parent and G.mustelinum as the donor parent.The study employed 2839 single nucleotide polymorphism(SNP)markers to mapping QTL associated with the resistance to VW based on phenotypic values.[Result]A total of 15 QTL related to the resistance to VW were detected,with 4.21%to 26.77% phenotypic variance explained (PVE). Additive effect analysis showed that 6 QTL had favorable alleles originating from G. mustelinum, while 9 QTL had favorable alleles originating from upland cotton B0011. Among these QTL, qVW-A01-1, qVW-A02-2 and qVW-A07-2 were detected in 2 or more environments, and the PVE ranged from 15.56% to 16.56%, 11.95% to 24.62% and 13.22% to 16.73%, respectively. Based on the best linear unbiased prediction (BLUP) value of resistance to VW in the BC5S5 population to re-linkage analysis, 5 QTL were detected. qVW-A01-1B and qVW-A02-1B were consistent with the physical positions of qVW-A01-1 and qVW-A02-2 detected by additive effect analysis, with PVE of 23.67% and 17.90%, respectively. [Conclusion] In this study, 2 stable QTL qVW-A01-1 and qVW-A02-2 were identified, which provide a basis for molecular marker-assisted selection breeding and functional identification of candidate gene in cotton breeding programs aimed at developing cotton resistance to VW.

关 键 词：陆地棉 抗病育种 黄萎病 数量性状位点 分子标记辅助育种 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]