The morphological diversity of pollen in the genus Gossypium  

作　　者：CAI Xiaoyan HOU Yuqing MUHAMMAD Umer Jawad WANG Heng XU Yanchao ZHENG Jie WANG Yuhong LIU Fang ZHOU Zhongli HUA Jinping WANG Kunbo 

机构地区：[1]State Key Laboratory of Cotton Biology,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,Henan,China [2]National Nanfan Research Institute(Sanya),Chinese Academy of Agriculture Sciences,Sanya 572025,Hainan,China [3]College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China

出　　处：《Journal of Cotton Research》2023年第1期48-59,共12页棉花研究（英文）

基　　金：This research was supported by the grants from the National Natural Science Foundation of China(32072023);the Project of Sanya Yazhou Bay Science and Technology City(SCKJ-JYRC-2022-88).

摘　　要：Background Plant pollen has diverse morphological characteristics that can be consistently passed down from generation to generation.Information on pollen morphology is thus immensely important for plant classification and identification.In the genus Gossypium,however,in-depth research on pollen morphology is lacking,with only few reports on limited cotton species.To evaluate the diversity of pollen in Gossypium,we therefore conducted a comprehensive analysis of the pollen morphology of 33 cotton species and varieties using scanning electron microscopy.Results The 33 analyzed cotton samples exhibited common pollen morphological features,including spherical shapes,radial symmetry,echination,panporation,and operculation,while the pollen size,spine shape,spine density and length showed distinctive features.Pollen size varied significantly among species,with diameters ranging from62.43 μm in G.harknessii to 103.41 μm in G.barbadense.The exine had an echinate sculptural texture,and spines were mostly conical or sharply conical but occasionally rod-like.Spine density varied from 173 in G.incanum to 54 in G.gossypioides,while spine length ranged from 3.53 μm in G.herbaceum to 9.47 μm in G.barbadense.In addition,the 33cotton species and varieties were grouped at a genetic distance of 3.83 into three clusters.Cluster Ⅰ comprised five allotetraploid AD-genome cotton species,four D-genome species,and one K-genome species.Cluster Ⅱ included 13diploid species from A,B,D,E,and G genomes,whereas Cluster Ⅲ only consisted one E-genome species G.incanum.Conclusions Although pollen characteristics alone are not enough to resolve taxonomic and systematic relationships within the genus Gossypium,our results add to knowledge on palynomorphology and contribute to phenological information on these taxa.Our findings should aid future systematic and phylogenetic studies of the Gossypium genus.

关 键 词：COTTON Scanning electron microscopy Pollen morphology DIVERSITY 

分 类 号：S562[农业科学—作物学]