五味藤茎皮高效液相色谱指纹图谱及数据挖掘研究  

HPLC Fingerprints and Data Mining of the Stem Bark of Securidacae Inappendiculatae Cortex

作　　者：韦玮 巫繁菁 黄大利 韦萍 潘广娟 李耀华[2] 包传红[2] WEI Wei;WU Fanjing;HUANG Dali;WEI Ping;PAN Guangjuan;LI Yaohua;BAO Chuanhong(Guangxi Orthopedic Traumatology Hospital,Nanning,Guangxi,China 530012;Guangxi University of Chinese Medicine,Nanning,Guangxi,China 530200)

机构地区：[1]广西骨伤医院,广西南宁530012 [2]广西中医药大学,广西南宁530200

出　　处：《中国药业》2023年第8期61-64,共4页China Pharmaceuticals

基　　金：广西壮族自治区中医药管理局自筹经费课题[GZZC2019002];广西中医药适宜技术开发与推广项目[GZSY21-08];广西中药优秀人才研修项目[桂中医药科教发[2022]14号];广西中医药大学自然科学研究青年基金课题[2015MS025];广西骨伤医院院级课题[GXGSZD-2019004]。

摘　　要：目的建立五味藤茎皮的高效液相色谱(HPLC)指纹图谱。方法采用HPLC法,色谱柱为Shim-pack VP-ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为327 nm(0~45 min)和260 nm(45~105 min),柱温为25℃,进样量为10μL。以1,7-二羟基?酮峰为参照峰,测定13批药材样品的HPLC指纹图谱,采用中药指纹图谱相似度评价系统(2012版)进行共有峰确定和相似度评价;并进行聚类分析和主成分分析。结果13批药材样品的HPLC图谱有10个共有峰,相似度为0.951~0.997。聚类分析和主成分分析结果显示,样品可分为两大类,主要区别在于贮存时间以4年为限,原因可能与购买和贮存时间有关。结论该指纹图谱及聚类分析和主成分分析结果,可为进一步科学评价五味藤茎皮质量提供参考。Objective To establish a high-performance liquid chromatography(HPLC)fingerprint of Securidacae Inappendiculatae Cortex(SIC).Methods HPLC method was adopted,the chromatographic column was Shim-pack VP-ODS column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.1%formic acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was set at 327 nm(0-45 min)and 260 nm(45-105 min),the column temperature was 25℃,and the injection volume was 10μL.The HPLC fingerprints of 13 batches of medicinal materials were determined with 1,7-dihydroxydione peak as the reference peak,and the Similarity Evaluation System for the Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Version)was used to determine the common peak and evaluate the similarity.Cluster analysis and principal component analysis were performed.Results There were 10 common peaks in the HPLC chromatograms of 13 batches of medicinal materials,and the similarity was in the range of 0.951-0.997.The results of cluster analysis and principal component analysis show that the samples could be clustered into two categories,with the main difference caused by the storage time(limited to four years),and its reason may be related to the purchase and storage time.Conclusion The results of the fingerprint,cluster analysis and principal component analysis can provide a reference for the scientific evaluation of the quality of SIC.

关 键 词：五味藤 高效液相色谱法 指纹图谱 聚类分析 主成分分析 

分 类 号：R917[医药卫生—药物分析学] R284.1[医药卫生—药学]