益糖康调控2型糖尿病大鼠脂肪组织TLR4/MyD88/NF-κB通路实验研究  

Experimental Research Mechanism on Regulating and Controling of TLR4/MyD88/NF-κB Pathway in Adipocytes of Type 2 Diabetic Rats by Yitangkang(益糖康)

作　　者：姜楠[1] 于嘉祥 马贤德[1] 杨宇峰[1] 冀天威[1] 张文顺 石岩[1] JIANG Nan;YU Jiaxiang;MA Xiande;YANG Yufeng;JI Tianwei;ZHANG Wenshun;SHI Yan(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属第二医院,辽宁沈阳110034

出　　处：《辽宁中医药大学学报》2023年第3期14-18,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB532004);辽宁省教育厅一般项目(L201941);中国博士后科学基金项目(2021M693853);辽宁省“兴辽英才计划”青年拔尖人才项目(XLYC2007121)。

摘　　要：目的观察益糖康对2型糖尿病大鼠脂肪组织TLR4/MyD88/NF-κB通路的影响,探讨益糖康对2型糖尿病的抗炎作用机制。方法将SPF级SD大鼠按照随机数字表法随机分为正常对照组(不做处理),模型组(蒸馏水,5 mL/kg),益糖康组(益糖康汤剂,5 g/kg)和二甲双胍组(150 mg/kg),每组10只。所有大鼠给药途径均为经口灌胃,每日1次,连续4周。干预4周后,称量各组大鼠的体质量,快速血糖仪测定各组大鼠空腹血糖(FPG),手工法测定各组大鼠血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量,称量白色脂肪(WAT)和棕色脂肪(BAT)总质量,并计算脂肪指数。酶联免疫吸附法检测肠黏膜组织中脂多糖(LPS)含量,脂肪组织中肿瘤坏死因子-α(TNF-α)含量,Western-blot法检测脂肪组织中Toll样受体4(TLR4)、髓样分化蛋白88(MyD88)、核转录因子-κB(NF-κB)蛋白表达水平。结果与正常对照组比较,其余3组大鼠的体质量,FPG、TG、TC、LDL-C、WAT、TNF-α含量,TLR4、MyD88、NF-κB表达水平均显著升高(P<0.01);HDL-C、BAT的水平均显著降低(P<0.01)。与模型组比较,益糖康组大鼠体质量显著升高(P<0.01),二甲双胍组没有显著差异(P>0.05),益糖康组和二甲双胍组大鼠FPG、TG、TC、LDL-C、WAT、TNF-α含量以及脂肪组织中TLR4、MyD88、NF-κB表达水平均显著降低(P<0.01),HDL-C、BAT的水平显著升高(P<0.01)。益糖康组大鼠体质量显著高于二甲双胍组(P<0.01)外,其余指标益糖康组和二甲双胍组之间比较均没有显著差异(P>0.05)。结论益糖康可能通过降低肠黏膜中LPS的含量进而抑制脂肪组织中TLR4/MyD88/NF-κB信号通路的过度活化,发挥抗炎作用,最终实现调控糖脂代谢的作用。Objective To observe the effect of Yitangkang(益糖康)on TLR4/MyD88/NF-κB pathway in adipocytes of type 2 diabetes rats,and to explore the anti-inflammatory mechanism of Yitangkang on type 2 diabetes.Methods SPF SD rats were randomly divided into normal control group,model group,Yitangkang group and metformin group according to random number table method,with 10 rats in each group.The intervention methods were normal control group(without treatment),model group(distilled water,5 mL/kg),Yitangkang group(Yitangkang Decoction,5 g/kg)and metformin group(150 mg/kg),respectively.All rats were administrated orally once a day for 4 weeks.After 4 weeks of intervention,the body weight of rats in each group was weighed,fasting blood glucose(FPG)was measured by fast glucose analyzer;the contents of triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)in serum of rats were determined by manual method;the total mass of white fat(WAT)and brown fat(BAT)were weighed and the fat index was calculated;the content of lipopolysaccharide(LPS)in intestinal mucosa was determined by enzyme-linked immunosorbent assay;tumor necrosis factor-α(TNF-α)content in adipose tissue;the expression levels of Toll-like receptor 4(TLR4),myeloid differentiation protein 88(MyD88)and nuclear transcription factor-κB(NF-κB)in adipose tissue were detected by Western-blot.Results Compared with normal control group,body weight,contents of FPG,TG,TC,LDL-C,WAT,TNF-αand expression levels of TLR4,MyD88 and NF-κB in the other three groups were significantly increased(P<0.01);the levels of HDL-C and BAT were significantly decreased(P<0.01).Compared with model group,body weight of Yitangkang group was significantly increased(P<0.01);there was no significant difference in metformin group(P>0.05).The contents of FPG,TG,TC,LDL-C,WAT,TNF-αand the expression levels of TLR4,MyD88 and NF-κB in adipose tissue of Yitangkang group and metformin group were significantly decreased(P<0.01).The le

关 键 词：益糖康 脂多糖 TLR4/MyD88/NF-κB通路 脂肪组织炎症 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]