柴胡治疗虚劳失眠潜在作用研究  

Study on Potential Function of Chaihu(Bupleuri Radix)in the Treatment of Insomnia Rats

作　　者：李艳凤[1,2] 刘雅舒 李艳生 刘征 LI Yanfeng;LIU Yashu;LI Yansheng;LIU Zheng(Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Key Laboratory of Basic and Application Research of Beiyao,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]教育部北药基础与应用研究重点实验室,黑龙江哈尔滨150040

出　　处：《辽宁中医药大学学报》2023年第3期31-34,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1707000);黑龙江省“头雁”团队项目。

摘　　要：目的分析含柴胡复方病证分布规律,统计分析并筛选柴胡潜在作用;动物实验验证柴胡治疗虚劳失眠的潜在作用。方法将48只SD大鼠按体质量随机分为正常组、模型组、中药对照组(固本延龄丸:813 mg/kg)、西药对照组(艾司唑仑片:0.18 mg/kg)、柴胡低剂量组和柴胡高剂量组(分别为北柴胡颗粒:90、360 mg/kg),每组8只。除正常组,其余各组进行跑台力竭训练,连续7 d,结束时和结束后24 h分别腹腔注射对氯苯丙氨酸(PCPA),350 mg/kg。除模型组,各组全程预防性灌胃给相应药物,记录每组体态特征,正常组给等容积蒸馏水。末次注射PCPA后禁食48 h,后处死取脑。ELISA试剂盒测定各组脑中5-羟色胺(5-HT)、4-氨基丁酸(GABA)含量。结果与模型组比较,其余大鼠脑中5-HT含量明显偏高(P<0.05),GABA含量明显偏低(P<0.05);与正常组比较,中药对照组5-HT明显升高(P<0.05),所有失眠大鼠GABA含量明显升高(P<0.05);与西药对照组比较,柴胡低剂量组GABA含量偏低(P<0.05)。结论柴胡有治疗虚劳失眠的作用,可能与其影响脑内单胺类神经递质与炎性反应因子含量有关。Objective To study the rule of distribution of decease of Chaihu(Bupleuri Radix),statistical analysis and selecting the potential function of Chaihu(Bupleuri Radix).Through animal experimentation confirmed that potential function of treating insomnia.Methods 48 SD rats were randomly divided into normal group,control group,Chinese medicine control group[Guben Yanling Pill(固本延龄丸):813 mg/kg],Western medicine control group(Estazolam tablets:0.18 mg/kg),Bupleurum low-dose group and Bupleurum high-dose group(Bupleurum granules:90 mg/kg,360 mg/kg respectively),each group has 8 rats.Except for the normal group,the rest of the groups received treadmill exhaustion training for 7 consecutive days,and PCPA was injected intraperitoneally at 350 mg/kg at the end and 24 hours after the end.Except for the control group,each group was given the corresponding drugs preventively throughout the entire course,and the posture characteristics of each group were recorded.The normal group was given equal volume of distilled water.Fasting for 48 hours after the last injection of PCPA,put them to death and take out the brain.By using the ELISA kit to determine the content of 5-HT and GABA in the brain of each group.Results Compared with the control group,the content of 5-HT in the brains of other rats was significantly higher(P<0.05),the content of GABA was significantly lower(P<0.05).Compared with the normal group,the 5-HT of the Chinese medicine control group was significantly increased(P<0.05),and the GABA content of all insomnia rats was significantly increased(P<0.05).Compared with the Western medicine control group,the GABA content of the Bupleurum low-dose group was lower(P<0.05).Conclusion Chaihu(Bupleuri Radix)has the effect of treating insomnia,which may be related to its influence on the content of monoamine neurotransmitters and inflammatory response factors in the brain.

关 键 词：柴胡 潜在作用 虚劳失眠 

分 类 号：R282.71[医药卫生—中药学] R256.23[医药卫生—中医学]