基于转录组分析不同食物水平对海月水母水螅体无性繁殖的影响  

Effects of different food supplies on the asexual reproduction of Aurelia coerulea based on transcriptome analysis

作　　者：朱见斌 王建艳 甄毓[1,2] 米铁柱[1,2] ZHU Jian-bin;WANG Jian-yan;ZHEN Yu;MI Tie-zhu(Key Laboratory of Marine Environment and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;Department of Science Research,Beijing Museum of Natural History,Beijing 100050,China)

机构地区：[1]中国海洋大学、海洋环境与生态教育部重点实验室,山东青岛266100 [2]中国海洋大学环境科学与工程学院,山东青岛266100 [3]北京自然博物馆科学研究部,北京100050

出　　处：《海洋环境科学》2023年第2期169-175,共7页Marine Environmental Science

基　　金：国家自然科学基金项目(41906112);北京市自然科学基金项目(8194059);国家重点研发计划课题项目(2017YFC1404404);北京市博士后科研活动经费项目(2016ZZ-85)。

摘　　要：为研究食物水平对海月水母螅状体无性繁殖能力及转录水平调控的影响,本文对不同食物水平培养下(F1组:25个卤虫/组/2天;F2组:250个卤虫/组/2天;F3组:1000个卤虫/组/2天)海月水母螅状体进行转录组分析,筛选差异表达基因,研究差异基因的富集通路,探讨了海月水母繁殖策略的分子机制。结果表明,15℃下匍匐茎出芽生殖是海月水母最主要的无性繁殖方式。食物水平越高,螅状体产生的子代个体(螅状体及碟状体)数量越多。转录组分析结果显示,F1组与F2组相比共有331个基因的表达水平呈显著性差异;F3组与F2组相比共有168个基因呈显著性差异。差异基因主要富集到两条与细胞生长、增殖、分化等相关的信号通路,分别为丝裂原活化蛋白激酶信号通路(mitogenactivated protein kinase,MAPK)和细胞周期信号通路(cell cycle)。随着食物水平升高,上述两条信号通路所涉及差异基因的表达对于海月水母无性繁殖均有促进作用。本研究结果有助于进一步认识海月水母无性繁殖的分子机制,为海月水母暴发的机理研究提供参考。To examine the molecular mechanism of Aurelia coerulea’s asexual reproductive strategy,transcriptome analysis was performed on polyps of A.coerulea under different food supplies(25,250 and 1000 newly hatched Artemia nauplius individuals per group every two days).We looked into the pathway where the differential gene enriched and screened the genes that were considerably expressed differently.Stolon budding was the most common type of asexual reproduction in A.coerulea polyps observed.The output of daughter polyps and ephyra increased considerably as food supplies increased.There were 331 genes that differed between the F1 and F2 groups,and 168 genes that differed between the F3 and F2 groups,according to transcriptome analysis.Two signal transduction pathways,MAPK(mitogen-activated protein kinase)and Cell cycle,were strengthened by the KEGG pathway analysis,which improved cell growth,proliferation and differentiation.The differentially expressed genes involved in the MAPK and Cell cycle signal pathways upregulated or down-regulated in response to increased food availability,speeding up the asexual reproduction.The findings of this study will help researchers better understand how A.coerulea reproduces asexually in response to food availability.

关 键 词：水母暴发 食物水平 无性繁殖 转录组 信号通路 

分 类 号：Q178.53[生物学—水生生物学]