曲古霉素A对脂多糖诱导的人微血管内皮细胞损伤和凋亡的影响  

Effect of trichostatin A on lipopolysaccharide-induced injury and apoptosis of human microvascular endothelial cell

作　　者：吴丰盛 刘瑜 刘海英 吴春远 余洁明 赵宏霞 Wu Fengsheng;Liu Yu;Liu Haiying;Wu Chunyuan;Yu Jieming;Zhao Hongxia(Pediatric Intensive Care Unit,Shenzhen Maternity and Children Healthcare Hospital Affiliated to Southern Medical University,Shenzhen 518028,China)

机构地区：[1]南方医科大学附属深圳妇幼保健院PICU,518028

出　　处：《中国小儿急救医学》2023年第3期203-207,共5页Chinese Pediatric Emergency Medicine

摘　　要：目的探讨组蛋白去乙酰化酶抑制剂曲古霉素A(TSA)对脂多糖(LPS)诱导的人微血管内皮细胞(HMEC)损伤和凋亡的影响。方法以HMEC为实验细胞,建立LPS诱导的脓毒症细胞模型,根据处理方式不同分为3组:空白对照组(加入磷酸盐缓冲液150μL)、LPS处理组(加入5μg/mL的LPS 150μL)、LPS+TSA干预组(加入5μg/mL的LPS和500μg/L的TSA 150μL)。各组细胞处理后继续培养24 h和48 h,ELISA法检测细胞培养液上清中乳酸脱氢酶(LDH)浓度并使用流式细胞术检测细胞凋亡程度,进行组间比较。结果与空白对照组比较,LPS处理组LDH浓度在24 h[(4.67±1.27)ng/L比(11.57±0.83)ng/L]和48 h[(7.93±0.80)ng/L比(12.72±0.89)ng/L]显著升高;与LPS处理组比较,LPS+TSA干预组LDH浓度在24 h[(6.01±0.29)ng/L]和48 h[(5.96±0.27)ng/L]明显下降,差异均有统计学意义(P<0.05)。与空白对照组相比,LPS处理组HMEC细胞凋亡率在24 h[(0.92±0.89)%比(1.66±0.09)%]和48 h[(1.09±0.14)%比(5.01±0.16)%]明显升高;与LPS处理组比较,LPS+TSA干预组HMEC细胞凋亡率在24 h[(1.36±0.01)%]和48 h[(4.19±0.23)%]有所下降,差异均有统计学意义(P<0.05)。结论TSA在脓毒症中具有降低细胞损伤和减少细胞凋亡的保护作用。Objective To investigate the effect of histone deacetylase inhibitor trichostatin A(TSA)on the lipopolysaccharide(LPS)-induced injury and apoptosis of human microvascular endothelial cell(HMEC).Methods HMECs were used as research cells to establish LPS-induced septic cell model,which were divided into three groups according to different treatments:control group(150μL of phosphate buffer),LPS group(150μL of 5μg/mL LPS),LPS+TSA group(150μL of 5μg/mL LPS and 500μg/L TSA).After cells of each group were cultured for 24 h and 48 h,the concentration of lactate dehydrogenase(LDH)in the culture supernatant was detected by enzyme-linked immunosorbent assay and the apoptosis rate of HMECs was detected by Annexin V-FTTC/PI staining,then comparison between different groups were made.Results Compared with the control group,LDH concentration in LPS group increased significantly at 24 h[(4.67±1.27)ng/L vs.(11.57±0.83)ng/L]and 48 h[(7.93±0.80)ng/L vs.(12.72±0.89)ng/L];Compared with LPS group,LDH concentration in LPS+TSA group decreased significantly at 24 h[(6.01±0.29)ng/L]and 48 h[(5.96±0.27)ng/L],and the differences were statistically significant(P<0.05).Compared with the control group,the apoptosis rates of HMEC cells in LPS group were significantly higher at 24 h[(0.92±0.89)%vs.(1.66±0.09)%]and 48 h[(1.09±0.14)%vs.(5.01±0.16)%];Compared with LPS group,the apoptosis rate of HMEC cells in LPS+TSA group significantly decreased at 24 h[(1.36±0.01)%]and 48 h[(4.19±0.23)%],the differences were statistically significant(P<0.05).Conclusion TSA has the protective effect of reducing cell injury and apoptosis in sepsis.

关 键 词：组蛋白去乙酰化酶抑制剂 曲古霉素A 脓毒症 细胞损伤 细胞凋亡 

分 类 号：R96[医药卫生—药理学]