去甲肾上腺素对犬腹主动脉瘤的影响  

Effects of norepinephrine on abdominal aortic aneurysm in dogs

作　　者：王瑶 徐在品[1] 孙安强[2] 李胤霆 明东东 李思南 洪晗 李欣妤 WANG Yao;XU Zaipin;SUN Anqiang;LI Yinting;MING Dongdong;LI Sinan;HONG Han;LI Xinyu(College of Animal Science,Guizhou University,Guiyang 550025,China;School of Biological and Medical Engineering,Beihang University,Beijing 100083,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]北京航空航天大学生物与医学工程学院,北京100083

出　　处：《中国兽医学报》2023年第3期546-552,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金地区基金资助项目(11862004);国家自然科学基金面上资助项目(11872096);黔科合平台人才基金资助项目([2018]5781)。

摘　　要：探讨不同剂量去甲肾上腺素(norepinephrine,NE)对犬腹主动脉瘤(abdominal aortic aneurysm,AAA)的影响。选取健康成年比格犬12只,雌性,体质量(11±1.5)kg,随机分为4组,每组3只,分别设NE低剂量组(0.2μg·kg^(-1)·min^(-1))、中剂量组(0.4μg·kg^(-1)·min^(-1))和高剂量组(0.8μg·kg^(-1)·min^(-1)),对照组每次只给予5%葡萄糖氯化钠注射液60 mL。手术分离腹主动脉并做血管插管,将弹力蛋白酶注射到腹主动脉插管段中膜,使注射部位形成AAA。在AAA形成后,静脉滴注不同剂量NE和5%葡萄糖氯化钠注射液,速度为60 mL/h,每天1 h,连续用药14 d。BL-420 N生物信号采集器监测AAA内血压变化,B超监测AAA直径及血流速度变化,计算机数值模拟血管壁面剪切应力(wall shear stress,WSS)变化;停药21 d后,牺牲试验犬,做HE和EVG切片染色观察AAA形态结构和细胞组成变化。结果显示,使用NE的各组收缩压、舒张压、平均动脉压与用药前相比极显著升高(P<0.01),血流速度与用药前相比显著或极显著增加(P<0.05或P<0.01);中剂量组和高剂量组AAA处的WSS极显著高于对照组和低剂量组(P<0.01);对照组腔内血栓沉积量、炎性细胞浸润程度、弹性纤维和弹力板层损害程度比使用NE的各组更严重。结果表明,NE可有效增加AAA内血压和血流速度,加大WSS,减少腔内血栓形成,在一定剂量范围内不会导致AAA破裂。The aim of this study is to investigate the effect of different doses of norepinephrine(norepinephrine,NE)on abdominal aortic aneurysm(abdominal aortic aneurysm,AAA)in dogs.Twelve healthy adult beagles,female,weighing(11±1.5)kg,were randomly divided into four groups with three dogs in each group,the NE low-dose group(0.2μg·kg^(-1)·min^(-1)),NE medium-dose group(0.4μg·kg^(-1)·min^(-1))and NE high-dose group(0.8μg·kg^(-1)·min^(-1))were set up respectively,the control group was only given 60 mL of 5%glucose sodium chloride injection each time.The abdominal aorta was surgically separated and intubated,then the elastase was injected into the middle membrane of the abdominal aorta to form AAA.After the formation of AAA,different dosage of NE and 5%glucose sodium chloride injection were administered intravenously at a rate of 60 mL/h,one hour a day for two weeks.Blood pressure changes within the AAA was monitored by BL-420 N biological signal collector,changes of AAA diameter and blood flow velocity were monitored by B ultrasound,wall shear stress(WSS)changes of blood vessels were by computer numerical simulation;after 21 d of drug withdrawal,the experimental dogs were sacrificed,HE and EVG sections were stained to observe the changes of AAA morphology and cell composition.The results were as follows:the systolic blood pressure,diastolic blood pressure and mean arterial pressure of each group using NE were significantly increased compared with those before medication(P<0.01),and the blood flow velocity was significantly or extremely significantly increased compared with those before medication(P<0.05or P<0.01);the WSS at the AAA in the middle-dose and high-dose groups was significantly higher than that in the control and low-dose groups(P<0.01);the amount of thrombus deposition,inflammatory cell infiltration,elastic fiber and elastic lamina damage in the control group were more serious than those in the groups using NE.The results indicated that the NE can effectively increase blood pressure and blood flow vel

关 键 词：去甲肾上腺素 腹主动脉瘤 形态结构 犬 

分 类 号：S854.5[农业科学—临床兽医学]