Sema4C、Erbin在宫颈癌中的表达及临床意义  

Expression and clinical significance of Sema4c and Erbin in cervical cancer

作　　者：杨叶 袁喜安 徐檬 颜彬 曹璜 胡俊波[2] 吴绪峰 马全富 YANG Ye;YUAN Xi'an;XU Meng;YAN Bin;CAO Huang;HU Junbo;WU Xufeng;MA Quanfu(Medical College of Wuhan University of Science and Technology,Wuhan 430062,Hubei,China;Maternal and Child Health Hospital of Hubei Province,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430070,Hubei,China;Hubei Clinical Medical Research Center for Gynecologic Malignancy,Wuhan 430070,Hubei,China)

机构地区：[1]武汉科技大学医学院,湖北武汉430062 [2]华中科技大学同济医学院附属湖北妇幼保健院,湖北武汉430070 [3]湖北省妇科恶性肿瘤临床医学研究中心,湖北武汉430070

出　　处：《武汉大学学报（医学版）》2023年第3期335-339,共5页Medical Journal of Wuhan University

基　　金：武汉中青年医学骨干人才培养工程(编号:武卫生计生[2016]59号)。

摘　　要：目的:探讨Sema4C、Erbin在宫颈癌组织中的表达及其与临床病理特征的关系。方法:应用多重荧光免疫组化方法检测126例宫颈癌组织及42例癌旁组织芯片中Sema4C、Erbin蛋白的表达。HeLa细胞中siRNA干扰Erbin后检测Sema4C及Erbin蛋白表达。结果:Sema4C及Erbin在宫颈癌组织主要定位于细胞质,Sema4C在癌及癌旁中的H-score平均值分别为154.78、125.38,差异有统计学意义(P=0.007);Erbin在癌及癌旁中的H-score平均值分别为189.71、168.62,差异有统计学意义(P=0.007);Sema4C与Erbin在宫颈癌组织中的表达呈正相关(r=0.742,P<0.01)。Sema4C在宫颈癌中的表达与年龄、临床分期相关(P分别为0.002和0.021),与病理分级、有无淋巴结受累、有无远处转移无关。Erbin在宫颈癌中的表达与上述临床特征均无关。HeLa细胞中siRNA抑制Erbin表达后Western Blot检测提示Sema4C表达下调。结论:Sema4C、Erbin在宫颈癌中的表达存在一定相关性,可能共同作用于宫颈癌的发生与进展。Objective:To investigate the expression of Sema4C and Erbin in cervical cancer and their relationship with clinicopathological features.Methods:The expression of Sema4C and Erbin proteins in 126 cases of cervical cancer tissues and 42 adjacent tissues were detected by multiplex fluorescent immunohistochemistry.Western Blot was used to detect the expression of Sema4C and Erbin in HeLa cells transfected with Erbin siRNA or control siRNA.Results:In cervical cancer tissues,Sema4C and Erbin were mainly localized in the cytoplasm.The mean H-score of Sema4C expression in 42 cervical cancer tissues and their adjacent tissues was 154.78 and 125.38,respectively,and the difference was statistically significant(P=0.007).The mean H-score of Erbin expression in 42 cervical cancer tissues and their adjacent tissues was 189.71 and 168.62,respectively,and the difference was statistically significant(P=0.007).Pearson correlation analysis showed that the expression of Sema4C protein in cervical cancer tissues was positively correlated with the expression of Erbin(r=0.742,P<0.O1).The expression of Sema4C in cervical cancer was correlated with age and clinical stage(P=0.002 and 0.021,respectively),but no correlation of Sema4C expression was found to the patho logical grade,lymph node involvement,and distant metastasis.The expression of Erbin in cervical cancer was not correlated with these clinicopathological features.Western Blot analysis indicated that Sema4C expression was down-regulated by siRNA targeting Erbin in HeLa cells.Conclusion:The expressions of Sema4C and Erbin in cervical cancer are correlated to some extent,which may play a role in the occurrence and progression of cervical cancer.

关 键 词：宫颈癌 Sema4C ERBIN 多重荧光免疫组化 

分 类 号：R711.74[医药卫生—妇产科学]