A shear-thinning,ROS-scavenging hydrogel combined with dental pulp stem cells promotes spinal cord repair by inhibiting ferroptosis  

作　　者：Yibo Ying Zhiyang Huang Yurong Tu Qiuji Wu Zhaoyu Li Yifan Zhang Huilei Yu Annian Zeng Hanzhi Huang Jiahui Ye Weiyang Ying Min Chen Zhiyi Feng Ziyue Xiang Qingsong Ye Sipin Zhu Zhouguang Wang 

机构地区：[1]School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou,Zhejiang,325000,China [2]Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,Zhejiang,China [3]Department of Pain Medicine,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,Zhejiang,China [4]Center of Regenerative Medicine,Renmin Hospital of Wuhan University,Wuhan,460030,Hubei,China [5]Massachusetts General Hospital,Harvard University,Boston,02114,USA [6]Department of Overseas Education College,Jimei University,Xiamen,Fujian,China

出　　处：《Bioactive Materials》2023年第4期274-290,共17页生物活性材料（英文）

基　　金：This study was partly funded by grants from the National Natural Science Funding of China(82172424,82271629);Outstanding Youth Fund of Zhejiang Province(LR22H060002);Zhejiang Medical and Health Science and Technology Plan Project(2022RC210,2021KY212);Wenzhou Basic Science Research Plan Project(Y20210045);CAMS Innovation Fund for Medical Sciences(2019-I2M-5-028).

摘　　要：Spinal cord injury(SCI)is a serious clinical disease.Due to the deformability and fragility of the spinal cord,overly rigid hydrogels cannot be used to treat SCI.Hence,we used TPA and Laponite to develop a hydrogel with shear-thinning ability.This hydrogel exhibits good deformation,allowing it to match the physical properties of the spinal cord;additionally,this hydrogel scavenges ROS well,allowing it to inhibit the lipid peroxidation caused by ferroptosis.According to the in vivo studies,the TPA@Laponite hydrogel could synergistically inhibit ferroptosis by improving vascular function and regulating iron metabolism.In addition,dental pulp stem cells(DPSCs)were introduced into the TPA@Laponite hydrogel to regulate the ratios of excitatory and inhibitory synapses.It was shown that this combination biomaterial effectively reduced muscle spasms and promoted recovery from SCI.

关 键 词：Shear-thinning hydrogel Dental pulp stem cells Ferroptosis ROS-Scavenging Synaptic regulation 

分 类 号：R651.2[医药卫生—外科学]