A versatile strategy for convenient circular bivalent functional nucleic acids construction  

作　　者：Xiao-Jing Zhang Zhuo Zhao Xia Wang Min-Hui Su Lili Ai Yingying Li Quan Yuan Xue-Qiang Wang Weihong Tan 

机构地区：[1]Molecular Science and Biomedicine Laboratory(MBL),State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,College of Biology,Aptamer Engineering Center of Hunan Province,Hunan University,Changsha 410082,China [2]Institute of Molecular Medicine(IMM),Renji Hospital,Shanghai Jiao Tong University School of Medicine,and College of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China [3]The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital),Hangzhou Institute ofMedicine(HIM),Chinese Academy of Sciences,Hangzhou 310022,China

出　　处：《National Science Review》2023年第2期141-151,共11页国家科学评论（英文版）

基　　金：supported by the National Key R&D Program of China(2018YFA0902300);the Huxiang Young Talent Program from Hunan Province(2019RS2022);the Science and Technology Project of Hunan Province(2019sk2201)。

摘　　要：Functional nucleic acids(FNAs),such as aptamers,nucleic acid enzymes and riboswitches play essential roles in various fields of life sciences.Tailoring of ingenious chemical moieties toward FNAs can enhance their biomedical properties and/or confer them with exogenic biological functions that,in turn,can considerably expand their biomedical applications,or even improve their clinical translations.Herein,we report the first example of a general chemical tailoring strategy that enables the divergent ligation of D NA sequences.By applying this technology,different types of aptamers and single-stranded nucleic acids of various lengths could be efficiently tailored to deliver the designed circular bivalent aptamers(CBApts)and cyclized DNA se quences with high yields.It is worth noting that CBApts exhibited significantly enhanced nuclease resistance,as well as considerably improved binding,targeting and tumor tissue enrichment abilities,which may pave the way for different investigations for biomedical purposes.

关 键 词：circular bivalent aptamers click chemistry chemical ligation 

分 类 号：R318[医药卫生—生物医学工程]