上转换纳米粒子-分子印迹复合荧光探针检测黄芩苷  

Upconversion nanoparticle-molecularly imprinted composite fluorescent probe for detection of baicalin

作　　者：衣佳琪 牛娜[1] 陈立钢[1] YI Jiaqi;NIU Na;CHEN Ligang(College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学化学化工与资源利用学院,哈尔滨150040

出　　处：《分析试验室》2023年第3期285-290,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21401019);黑龙江省自然科学基金(B2008001)资助。

摘　　要：构建了一种近红外激发上转换纳米粒子-分子印迹(UCNPs@MIPs)复合荧光探针,并应用于尿液中黄芩苷的检测。以黄芩苷作为模板分子,采用溶胶凝胶技术将SiO2包覆的上转换纳米粒子封装到分子印迹聚合物中。当模板分子被去除后,该复合材料留下特异性结合位点,与黄芩苷之间可产生静电引力相互作用。黄芩苷可使上转换纳米粒子发生荧光猝灭实现对其选择性荧光分析。最优条件下,黄芩苷的浓度在0.1~50μmol/L范围线性良好,检出限为0.03μmol/L,尿液样品的加标回收率为94.0%~100.5%,相对标准偏差为1.3%~3.9%。该方法可实现对黄芩苷的选择性检测。A near-infrared excited upconversion nanoparticle-molecularly imprinted(UCNPs@MIPs) composite fluorescent probe was constructed and applied to detect baicalin in urine. The sol-gel technique was used to encapsulat silica-coated upconversion nanoparticles into molecularly imprinted polymers. When the template molecule was removed, the composite left specific binding sites that could generate electrostatic gravitational interactions with baicalin. In addition, baicalin can quench the fluorescence of the upconversion nanoparticles, thus enabling their selective fluorescence analysis. Under the optimal conditions, good linearity was obtained in the concentration range of 0.1-50 μmol/L, and the detection limit was 0.03 μmol/L. The spiked recoveries of urine samples were 94.0%-100.5% with the relative standard deviations of 1.3%-3.9%. The experimental results showed that the method could achieve the selective detection of baicalin.

关 键 词：上转换纳米粒子 分子印迹 荧光猝灭 黄芩苷 尿液 

分 类 号：O657.3[理学—分析化学]