双频超声辅助酶解同时提取生菜中的4种重金属离子  

Dual-frequency ultrasound-assisted enzymatic hydrolysis for simultaneous extraction of four heavy metals ions lettuce

作　　者：袁敏[1] 尹佳琪 丰东升 韩奕奕 杨晓君 马颖清 王博 曹慧[1] 徐斐[1] YUAN Min;YIN Jiaqi;FENG Dongsheng;HAN Yiyi;YANG Xiaojun;MA Yingqing;WANG Bo;CAO Hui;XU Fei(Shanghai Engineering Research Centre for Food Rapid Detection,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai Center of Agri-products Quality and Safety,Shanghai 201708,China;Shanghai Municipal Supervisory Institute Veterinary Drugs and Feedstaff,Shanghai 201103,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海食品快速检测工程技术研究中心,上海200093 [2]上海市农产品质量安全中心,上海201708 [3]上海市兽药饲料检测所,上海201103

出　　处：《分析试验室》2023年第3期319-323,共5页Chinese Journal of Analysis Laboratory

基　　金：上海市农业农村委员会科技兴农项目(2020-02-08-00-07-F01477);上海市科委重点攻关项目(18391901200)资助。

摘　　要：提出了一种一步提取4种重金属离子的新方法。基于双频超声辅助酶解技术,高强度的超声探头、超声波水浴控温技术与组合酶相结合,可从生菜中高效同步提取4种重金属离子。实验结果表明,纤维素酶和风味蛋白酶的组合酶更有利于重金属离子的提取。双频超声模式下,630 W功率和50℃水浴温度显著提高了生物分子的酶解率,只需15 min即可同时从生菜中提取出As^(3+), Cd^(2+), Hg^(2+), Pb^(2+)等4种重金属离子,且回收率达98.3%~106.0%,相对标准偏差小于6%。双频超声辅助酶解与生物传感检测技术相结合,可实现生菜中4种重金属离子的同时提取和检测。A new extraction method for the four heavy metal ions, including As^(3+), Cd^(2+), Hg^(2+)and Pb^(2+), by one step was proposed. Based on dual-frequency ultrasonic-assisted enzymatic hydrolysis technology, high-intensity ultrasonic probe, ultrasonic water bath temperature control technology and combinative enzymes were combined to extract four metal ions efficiently and synchronously from lettuces. Experimental results show that combinative enzyme of cellulase and flavorzyme was more beneficial to the extraction of heavy metal ions. Under dualfrequency ultrasound mode, and the conditions of 630 W power and 50℃ water bath temperature, the enzymatic hydrolysis rate of biomolecules was significantly improved. Four heavy metal ions can be simultaneously extracted from lettuce in only 15 min. The recoveries of four heavy metal ions were 98.3%-106.0%, and the relative standard deviations were less than 6%. The combination of dual-frequency ultrasonic-assisted enzymatic hydrolysis and biosensing detection technology can realize the simultaneous extraction and detection of four heavy metal ions in lettuces.

关 键 词：双频超声辅助酶解 提取 重金属离子 

分 类 号：O652.6[理学—分析化学]