单分子力谱测量主体葫芦[7]脲和客体分子间的结合力  

Measurement of binding forces between host cucurbit[7]uril and guest molecules by single-molecule force spectroscopy

作　　者：李昊 张明阳 钱功明 李云川 郭靖 LI Hao;ZHANG Mingyang;QIAN Gongming;LI Yunchuan;GUO Jing(The State Key Laboratory of Refractories and Metallurgy,College of Materials and Metallurgy,Wuhan University of Science and Technology,Wuhan 430081,China)

机构地区：[1]武汉科技大学材料与冶金学院,耐火材料与冶金国家重点实验室,武汉430081

出　　处：《分析试验室》2023年第3期384-391,共8页Chinese Journal of Analysis Laboratory

摘　　要：使用单分子力谱(SMFS)研究了大环主体分子中主体葫芦[7]脲与带有苯并噻唑、苯并咪唑和苯并恶唑基团的3个客体之间的相互作用,并在不同的加载速率下对3种主客体系的相互作用力进行了测量比较。研究了不同pH下3种客体分子与葫芦[7]脲相互作用力的变化,结果显示,质子化的客体分子与葫芦[7]脲的主客体相互作用力小于非质子化的客体分子与葫芦[7]脲的主客体相互作用力。结合核磁质谱分析和单分子力谱数据,推测疏水相互作用对结合力起着重要作用。研究结果有助于合理设计和构建为抗癌药物递送提供可能的智能纳米载体超分子。The interactions between host cucurbit[7]uril(CB[7]) and three guests with benzothiazole, benzimidazole and benzoxazole groups in macrocyclic host molecules were studied by single-molecule force spectroscopy(SMFS), and the interaction forces of three host-guest systems were measured and compared under different loading rates. The changes of interaction forces between three kinds of guest molecules and cucurbit[7]uril under different pH were studied. The results showed that the interaction forces between protonated guest molecules and cucurbit[7]uril were less than that between non-protonated guest molecules and cucurbit[7]uril.Based on the analysis of nuclear magnetic mass spectrometry and the data of single-molecule force spectra, it was speculated that hydrophobic interaction played an important role in the binding force. The results were helpful for the rational design and construction of possible supramolecules as intelligent nanocarriers for anticancer drug delivery.

关 键 词：单分子力谱 主客体作用力 脲 

分 类 号：R979.19[医药卫生—药品]