骨置入物表面仿生矿化微纳拓扑结构载碘涂层的抗菌与成骨性能研究  

Antibacterial and osteogenic properties of biomimetic mineralized iodine-loaded coating with micro-nano topography on bone implants

作　　者：王翊凯 马思远 金志辉 陈森[1] 叶佳[1] 聂志刚[1] 王梦玮 曹家睿 任义军 Wang Yikai;Ma Siyuan;Jin Zhihui;Chen Sen;Ye Jia;Nie Zhigang;Wang Mengwei;Cao Jiarui;Ren Yijun(Microorthopedics/Trauma Center,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院显微骨科/创伤中心,武汉430060

出　　处：《中华创伤骨科杂志》2023年第3期260-266,共7页Chinese Journal of Orthopaedic Trauma

基　　金：中央高校基本科研业务费专项资金资助项目(2042022kf1076);湖北省自然科学基金面上项目(2020CFB659);湖北省卫生健康委员会面上项目(WJ2021M139)。

摘　　要：目的探讨骨置入物表面仿生矿化微纳拓扑结构载碘涂层的抗菌与成骨性能。方法首先,在钛合金(Ti6Al4V)片表面通过碱热反应构建钛酸氢钠的纤维网状结构,标记为碱蚀(AT)组;然后,在改良模拟体液中仿生矿化形成高比表面积的微纳拓扑结构,标记为碱蚀后仿生矿化(AT-CaP)组;最后,负载聚乙烯吡咯烷酮碘(PVPI)构建新型抗菌骨整合涂层,标记为碱蚀后仿生矿化载碘(AT-CaP-PVPI)组。每组3次平行实验,在涂层表面观察金黄色葡萄球菌的形貌和数量,检测其体外抗菌性能。取15只SD雄性大鼠,随机分为3组(n=5):AT组、AT-CaP组、AT-CaP-PVPI组,在大鼠股骨下端髓腔内注射金黄色葡萄球菌后,分别置入经AT、AT-CaP及AT-CaP-PVPI涂层处理的钛棒。比较3组大鼠术后4周股骨置入物表面的成骨状态、新生骨量体积比及骨小梁数目等。结果体外实验结果显示:AT组和AT-CaP组涂层表面细菌保持其固有的椭圆球形或圆球形,细菌处于良好的存活状态;而AT-CaP-PVPI组涂层表面细菌出现膜变形凹陷,有些细菌已经完全破裂、溶解,大量细菌死亡。体内实验结果显示:AT组置入物周围几乎无新生骨形成;AT-CaP组置入物周围散在分布新生骨,但是新生骨分布不连续;AT-CaP-PVPI组置入物周围可见大量新生骨,且新生骨分布均匀,无感染迹象。AT-CaP-PVPI组置入物表面新生骨量体积比和骨小梁数目分别为0.453±0.206和6.055±0.536,均显著高于AT组(0.046±0.028、1.667±1.249)和AT-CaP组(0.188±0.052、3.804±0.889),差异均有统计学意义(P<0.05)。结论置入物表面仿生矿化微纳拓扑结构载碘涂层具有较强的抗菌能力和较好的骨整合活性。Objective To investigate the antibacterial and osteogenic properties of biomimetic mineralized iodine-loaded coating with micro-nano topography on the surface of bone implants.Methods After the fiber network structure of sodium hydrogen titanate was constructed by alkali thermal reaction on the surface of Ti6Al4V(noted as AT),it was biomimetically mineralized in the modified simulated body fluid to form a micro-nano topology with high specific surface area(noted as AT-CaP),and finally loaded with PVPI to construct a novel antibacterial osseointegration coating(noted as AT-CaP-PVPI).The study was conducted in AT,AT-CaP,and AT-CaP-PVPI groups,in each of which 3 parallel experiments were performed.The morphology and colony counting of Staphylococcus aureus on the coating surface were observed to detect the in vitro antibacterial performance of the coating.Fifteen male SD rats were randomly divided into 3 groups(n=5):AT,AT-CaP,and AT-CaP-PVPI.After intramedullary injection of Staphylococcus aureus into the lower end of the femur in the SD rats,titanium rods coated with AT,AT-CaP,and AT-CaP-PVPI were inserted into the marrow cavity.The osteogenesis,volume ratio of new bone mass and number of trabeculae on the surface of the femoral implants were compared between the 3 groups 4 weeks after operation.Results In AT and AT-CaP groups,a large number of bacteria grew in their inherent elliptical or spherical shape on the implant surface and a large number of colonies were seen on the plate;in AT-CaP-PVPI group,the bacteria on the coating surface exhibited membrane deformation and depression,some of them were completely broken and dissolved,and a large number died.There was almost no new bone formation around the implants in AT group;new bone scattered around the implants with discontinuous distribution in AT-CaP group;a great amount of new bone was seen around the implants with even distribution but no signs of infection in AT-CaP-PVPI group.The volume ratio of new bone mass and the number of trabeculae on the implant surfa

关 键 词：骨疾病 感染性 置入物 碘 抗菌涂层 骨整合 

分 类 号：R318.08[医药卫生—生物医学工程]