Dopamine-mimetic-coated polyamidoamine-functionalized Fe_(3)O_(4) nanoparticles for safe and efficient gene delivery  

作　　者：Liang Liu Chaobing Liu Zhaojun Yang Yiran Chen Xin Chen Jintao Guan 

机构地区：[1]College of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China [2]College of Chemistry and Environmental Engineering,Wuhan Polytechnic University,Wuhan 430023,China

出　　处：《Frontiers of Materials Science》2023年第1期141-152,共12页材料学前沿（英文版）

基　　金：This work was supported in part by the National Natural Science Foundation of China(Grant No.21602166);the Natural Science Foundation of Hubei Province(Grant No.2020CFB760);the Research and Innovation Initiatives of WHPU(Grant No.2021Y11).

摘　　要：Fe_(3)O_(4) nanoparticles(NPs)are widely used in the construction of drug and gene delivery vectors because of their particular physicochemical properties.Surface modification can not only reduce the cytotoxicity of Fe_(3)O_(4),but also further improve the biocompatibility and delivery efficiency.In this work,firstly,polydopamine(PDA)-coated Fe_(3)O_(4) NPs(named Fe_(3)O_(4)@PDA)were prepared by using the self-polymerization characteristics of dopamine in alkaline environment.Then,polyamidoamine(PAMAM)was modified by the Michael addition reaction to prepare water-soluble core shell magnetic NPs of Fe_(3)O_(4)@PDA@PAMAM,and its potential as gene vector was further evaluated.The results revealed that Fe_(3)O_(4)@PDA@PAMAM had the ability to condense and protect DNA,and showed lower cytotoxicity,higher cell uptake and transfection efficiency than those of PAMAM.It has the potential to become a magnetic targeted gene vector for further study.

关 键 词：gene vector nanoparticle POLYDOPAMINE gene transfection 

分 类 号：TB3[一般工业技术—材料科学与工程]