不同时间点㨰法按摩器对骨骼肌损伤家兔炎症反应的影响  

Effects of different time point rolling massager on inflammation in rabbits with skeletalmuscle injury

作　　者：薛惠天 王兰兰 孙梦龙 黄博 阮磊 彭亮[1] Xue Huitian;Wang Lanlan;Sun Menglong;Huang Bo;Ruan Lei;Peng Liang(College of Acupuncture&Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,长沙410208

出　　处：《国际中医中药杂志》2023年第4期433-438,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82174521);湖南中医药大学研究生创新课题项目(2019CX38)。

摘　　要：目的观察不同时间点㨰法按摩器对骨骼肌损伤家兔局部组织形态,以及骨骼肌组织及血清中TNF-α、IL-1β水平的影响,探究㨰法作用于骨骼肌损伤的时间效应机制。方法72只新西兰兔按随机数字表法分为空白组、模型组、㨰法治疗组,每组24只,每组按损伤时间点分为1、3、5、7、9、11 d亚组,每组4只。模型组、㨰法治疗组采用钝挫伤法建立股四头肌损伤模型。㨰法治疗组各亚组采用自制㨰法按摩器进行㨰法干预3 d,2次/d,3 min/次。干预完成后24 h,采用HE染色观察骨骼肌形态学变化,ELISA法检测血清和受损骨骼肌中TNF-α、IL-1β水平。结果与空白组比较,模型组炎症细胞浸润明显,水肿严重,肌纤维断裂;1 d㨰法治疗组炎症细胞浸润加剧,肌细胞凋亡,肌纤维断裂坏死更严重;7 d㨰法治疗组炎症明显改善,和正常健康肌肉组织相差较小。造模后,3 d模型组骨骼肌组织中TNF-α和IL-1β水平及血清TNF-α水平高于1 d模型组(P<0.05)。与空白组比较,模型组各亚组及㨰法治疗组各亚组骨骼肌组织及血清中TNF-α和IL-1β水平升高(P<0.01);1 d㨰法治疗组骨骼肌组织TNF-α和IL-1β水平及血清TNF-α水平高于1 d模型组,3、5、7、9、11 d㨰法治疗组骨骼肌及血清TNF-α和IL-1β水平低于模型组亚组(P<0.05)。与7 d㨰法治疗组比较,1、3、5 d㨰法治疗组骨骼肌和血清IL-1β及血清TNF-α水平升高(P<0.05),1、3 d㨰法治疗组骨骼肌TNF-α水平升高(P<0.05)。结论家兔骨骼肌损伤后1 d处于急性炎症期,此时不适宜用㨰法治疗;损伤后7 d开始实施㨰法治疗可减轻炎症反应,加快骨骼肌修复速度,提高功能恢复质量。Objective To observe the effects of rolling method massager on local tissue morphology,tissue and serum TNF-αand IL-1βin rabbits with skeletal muscle injury at different time points;To investigate the mechanism of temporal effect of rolling method action on skeletal muscle injury.Methods Totally 72 New Zealand rabbits were divided into blank group,model group and rolling method treatment group according to random number table method,with 24 rabbits in each group.Rabbits in each group were divided into 1 d,3 d,5 d,7 d,9 d and 11 d subgroups according to the time point of injury,with 4 rabbits in each group.Blunt contusion was used to model the model group and the rolling method treatment group. Each subgroup of therolling method treatment group was subjected to rolling method intervention for 3 d, using ahomemade rolling method massager, 2 times/d, 3 min/time. At 24 h after the completion of theintervention, the histomorphological changes were observed by HE staining, and the TNF- α andIL-1β contents in serum and damaged skeletal muscle tissues were detected by ELISA.Results Compared with the blank group, the inflammatory cell infiltration in the model group wasobvious, edema was severe, and myofibers were broken;the inflammatory cell infiltration in the 1 drolling method treatment group was intensified, myocytes were apoptotic, and myofibers werebroken and necrosed more seriously;the inflammation in the 7 d rolling method treatment groupwas obviously improved with the best effect, and the difference with normal healthy muscle tissuewas smaller. After modeling, TNF- α and IL-1β levels in skeletal muscle tissues and serum TNF- αlevels were higher in the 3 d model group than in the 1 d model group (P<0.05). Compared with theblank group, TNF-α and IL-1β levels in skeletal muscle tissues and serum increased in each subgroupof the model group and each subgroup of the rolling method treatment group (P<0.01);Comparedwith the 1 d model group, TNF-α and IL-1β levels in skeletal muscle tissues and serum TNF-α le

关 键 词：滚法推拿疗法 骨骼肌损伤 炎症 肿瘤坏死因子-Α 白细胞介素-1Β 

分 类 号：R244.1[医药卫生—针灸推拿学]