基于网络药理学分析金胆片、消炎利胆片和熊去氧胆酸治疗胆结石胆囊炎作用机制  

Analysis on mechanisms of Jindan Tablets, Xiaoyan Lidan Tablets and ursodeoxycholic acid inthe treatment of gallstones and cholecystitis based on network pharmacology

作　　者：韩旭 王娟 郭洪涛[2] 赵宁[1] 崔赵丽[3] 丁治国[4] 顾浩[1] 姜淼[1] Han Xu;Wang Juan;Guo Hongtao;Zhao Ning;Cui Zhaoli;Ding Zhiguo;Gu Hao;Jiang Miao(Center of Traditional Chinese Medicine Petislence Disease,Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Department of Rheumatology and Immunology,The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China;Department of Endocrinology,East District of Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 101100,China;Department of Surgery,East District of Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 101100,China)

机构地区：[1]中国中医科学院中医临床基础医学研究所中医药疫病研究中心,北京100700 [2]河南中医药大学第一附属医院风湿免疫科,郑州450000 [3]北京中医药大学东直门医院东区内分泌科,北京101100 [4]北京中医药大学东直门医院东区外科,北京101100

出　　处：《国际中医中药杂志》2023年第4期464-471,共8页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81873181、81603401);中央级公益性科研院所基本科研业务费专项资金资助项目(Z0659、Z0647);北京市科技计划课题资助项目(Z121100000312006)。

摘　　要：目的基于网络药理学方法分析金胆片、消炎利胆片和熊去氧胆酸治疗胆结石胆囊炎的作用机制,并进行比较分析。方法检索TCMSP、中药分子机理的生物信息学分析工具(BATMAN-TCM),收集金胆片、消炎利胆片和熊去氧胆酸化学成分及靶点,使用DAVID 6.8数据库检索药物靶点的关联疾病。使用GeneCards等数据库检索胆结石、胆囊炎的疾病靶点,基于3种药物及2种疾病的交集靶点建立PPI网络,采用DAVID 6.8数据库进行KEGG通路富集分析。运用Cytoscape 3.7.1软件构建“中药-成分-靶点”网络,并进行拓扑分析。结果收集金胆片化学成分222个,药物靶点3133个;消炎利胆片化学成分104个,作用靶点1425个;熊去氧胆酸片化学成分1个,作用靶点119个。3种药物与31种疾病相关联。收集胆结石胆囊炎的疾病靶点1382个。3组药物治疗胆结石胆囊炎的作用靶点分别为237个、163个和33个,其中3种药物共有作用靶点17个,金胆片与消炎利胆片共有作用靶点20个。3种药物作用靶点KEGG通路富集显示,分别得到113个、74个和10个显著性KEGG富集通路。结论3种药物治疗胆结石胆囊炎,均具有调节代谢、抑制炎症反应的功能,同时参与细胞凋亡、氧化应激和癌症病变过程。但金胆片偏重参与癌症病程和抑制炎症,并可促进血管生成;消炎利胆片和熊去氧胆酸侧重调节胆固醇代谢,且消炎利胆片还可调节类固醇代谢和抑制炎症,熊去氧胆酸可调节胆汁酸代谢。Objective To analyze the mechanism of Jindan Tablets,Xiaoyan Lidan Tablets and ursodeoxycholic acid in the treatment of gallstone and cholecystitis based on network pharmacology;To conduct a comparative analysis.Methods The chemical components of Jindan Tablets,Xiaoyan Lidan Tablets and ursodeoxycholic acid and their drug targets were collected from Traditional Chinese Medicine Database and Analysis Platform(TCMSP).DAVID 6.8 database was used to search for the associated diseases of the drug targets.The disease targets of gallstone and cholecystitis were collected from GeneCards and other databases.The protein-protein interactions network was established based on the intersecting targets of three drugs and two diseases.KEGG enrichment analysis was performed based on the DAVID 6.8 database.Cytoscape 3.7.1 software was used to construct a complex network and topology analysis of component-target-disease between three drugs and diseases.Results 222 chemical components and 3133 drug targets were collected for Jindan Tablets.104 chemical components and 1425 action targets were collected for Xiaoyan Lidan Tablets.1 chemical component and 119 action targets were collected for ursodeoxycholic acid.The three drugs were associated with 31 diseases.1382 disease targets for gallstones and cholecystitis were collected.There were 237,163 and 33 targets for gallstones and cholecystitis in the three drugs,of which 17 were shared by the three drugs and 20 were shared by Jindan Tablets and Xiaoyan Lidan Tablets.Based on the DAVID database,113,74 and 10 significant KEGG enrichment pathways were obtained for the three drugs respectively.Conclusions The three drugs shared many targets and pathways in the treatment of gallstones and cholecystitis,which all had the function of regulating metabolism and inhibiting inflammatory response,while participating in apoptosis,oxidative stress and cancer pathology process.However,they had their own special effects,with Jindan Tablets favoring involving in the cancer process and inhibition of inf

关 键 词：胆结石 胆囊炎 金胆片 消炎利胆片 熊去氧胆酸 网络药理学 

分 类 号：R285[医药卫生—中药学]