Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface  

作　　者：Qingsong Jiang Liren Wang Zhanhong Liu Jinlei Su Yajun Tang Peijie Tan Xiangdong Zhu Kai Zhang Xing Ma Jia Jiang Jinzhong Zhao Hai Lin Xingdong Zhang 

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,29 Wangjiang Road,Chengdu,Sichuan,China [2]Department of Sports Medicine,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital,600 Yishan Road,Shanghai,China [3]Department of Orthopaedics,First Affiliated Hospital of Xi’an Jiaotong University,277 West Yanta Road,Xi’an,Shaanxi,China

出　　处：《Bioactive Materials》2023年第2期1-15,共15页生物活性材料（英文）

基　　金：supported by the National Key Research and Development Program of China(2018YFC1106200,2018YFC1106203);the National Natural Science Foundation of China(32071330);the Sichuan Science and Technology Innovation Team(2019JDTD0008);the Key Science and Technology Program of Guangxi Province(AA17204085-2).

摘　　要：Healing of an anterior cruciate ligament(ACL)autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue,which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failure recurrence.In this study,an injectable hydroxyapatite/type I collagen(HAp/Col I)paste was developed to construct a suitable local microenvironment to accelerate the healing of bone-tendon interface.Physicochemical characterization demonstrated that the HAp/Col I paste was injectable,uniform and stable.The in vitro cell culture illustrated that the paste could promote MC3T3-E1 cells proliferation and osteogenic expression.The results of a canine ACL reconstruction study showed that the reconstructive ACL had similar texture and color as the native ACL.The average width of the tunnel,total bone volume,bone volume/tissue volume and trabecular number acquired from micro-CT analysis suggested that the healing of tendon-bone interface in experimental group was better than that in control group.The biomechanical test showed the maximal loads in experimental group achieved approximately half of native ACL’s maximal load at 24 weeks.According to histological examination,Sharpey fibers could be observed as early as 12 weeks postoperatively while a typical four-layer transitional structure of insertion site was regenerated at 48 weeks in the experimental group.The injectable HAp/Col I paste provided a biomimetic scaffold and microenvironment for early cell attachment and proliferation,further osteogenic expression and extracellular matrix deposition,and in vivo structural and functional regeneration of the tendon-bone interface.

关 键 词：Anterior cruciate ligament reconstruction Tendon-bone healing Injectable paste HYDROXYAPATITE COLLAGEN 

分 类 号：R68[医药卫生—骨科学]